FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004949959

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004949959
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3325128
Sequences flagged as poor quality0
Sequence length8
%GC62

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCAAGGA322584097.01400968624365No Hit
GGCAAGGC169010.5082811849649096No Hit
GGCAAGAA102980.3097023633375918No Hit
GGCAACGA87720.2638093932023068No Hit
GGCAAGCA75680.2276002608019902Clontech Universal Primer Mix Long (100% over 8bp)
GCCAAGGA60930.18324106620857902RNA PCR Primer, Index 1 (100% over 8bp)
CGCAAGGA55710.16754242242704642No Hit
GGAAAGGA53690.16146746832001654No Hit
GGCAGGGA47930.14414482690591157No Hit
GGGAAGGA35590.10703347359861035No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)