FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004949975

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004949975
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences870064
Sequences flagged as poor quality0
Sequence length16
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTAGTGAACGTAA44860.5155942551352545No Hit
CGACCAGACGCGCTGT41160.4730686478236084No Hit
TAGCTTTACCCTATCC38330.44054230493388996No Hit
CCAGGACTGTAACTCG31040.35675536512256567No Hit
ATCTGTACTGGGACTA28060.32250501112561836No Hit
CTTCCTTTGGCTCAGC26180.30089740524834957No Hit
GTTCTCGACCGTAGAG26040.2992883282149359No Hit
CGATCAAACCCGGCTA24950.2867605141690726No Hit
GGGGGGGGGGGGGGGG22940.26365876533220545No Hit
AGACCATTGAATCCAC22380.2572224571985509No Hit
GGCAACAGAATAGCAA22240.2556133801651373No Hit
TGCTGCTACTTTCTTG21610.24837253351477595No Hit
ACAGCCGACTTGCGTA21010.24147648908586036No Hit
GATGAATGACTCCTAC18830.21642086099413377No Hit
AACTTACCTCACCTAA18750.2155013884036117No Hit
ATCGAAAGATTTCCGC17940.20619172842457567No Hit
CGCGTTGGAGCGGTTC17600.20228396991485684No Hit
CCCTGAACTAGCTTTG16360.18803214476176464No Hit
AGTGGAGTGAGTTGAA16050.1844691884734916No Hit
GTAACTGTGTGAAAGT16040.18435425439967634No Hit
CAATTCGGATAACGGG15490.17803288033983708No Hit
GGGTAGAACCAGTCTA14520.1668842751797569No Hit
AAGAACCCTTAACCCA14200.16320638481766858No Hit
TTTAGTCTGAGCAATA14190.16309145074385334No Hit
TTCCTAAACTAATGCC14040.16136743963662442No Hit
GTGAAGCGATCCCAGG13190.15159804336232738No Hit
CGCGATCGATATCTGG13120.15079350484562057No Hit
TCCGGGAACGTTCTCC13090.15044870262417478No Hit
TGATAGAGAGACTGTT12720.14619614189301017No Hit
TGGAACACTCCTAGTA12680.14573640559774914No Hit
TGCAGATACTGCCACG12380.14228838338329136No Hit
GCAACTTGAAGGTGCA12330.14171371301421504No Hit
CTTTAACTGTCCGTAA12140.1395299656117251No Hit
AGTCCTGCTCGAACGA12050.13849555894738777No Hit
AGGTGTGGATCATGTG11900.13677154784015888No Hit
CACATCCTGAGCCTAT11890.13665661376634364No Hit
CTATTGGGACTTGTGA11850.13619687747108258No Hit
CTTAGATCTTGTCACA11770.1352774048805605No Hit
CATAGGTACAAGGTGC11660.13401313006859264No Hit
TACGTTTACTGCACCG11650.1338981959947774No Hit
TGGCAATTGAGTGGTG11550.13274885525662478No Hit
ATTTCTGACGGGTAGT11520.13240405303517902No Hit
TCTCAGAGATGCATGT11510.13228911896136375No Hit
TGCAGTATGACACCGC11490.13205925081373324No Hit
GAGTGTTACACCTGCG11430.13136964637084167No Hit
TCAAAGCACTCTGCAA11080.12734695378730762No Hit
ATGTACGTGTACTTTC11050.12700215156586184No Hit
AGAGTACCTTAATCGC10890.12516320638481768No Hit
TGAGGGATGTACGTTG10850.12470347008955664No Hit
GGTCTAAACTCCTACC10600.12183011824417514No Hit
TTCTTAGACGTCCCGA10600.12183011824417514No Hit
GCATGATACTTGCGTA10430.11987623898931574No Hit
TCTATGGGATGCTCAA10430.11987623898931574No Hit
GGGTGTGCTCGCAAAG10400.11953143676786994No Hit
TGAACTGTGAACGTAA10380.11930156862023944No Hit
TCCATTTGAAATCTGC10370.11918663454642416No Hit
GGTCGCATGGTACTAC10340.11884183232497839No Hit
TACTGTTTGCTTAAGC10150.11665808492248846No Hit
GGGATGTGACTGGAAG10130.11642821677485793No Hit
CACCAGGTGCGCCACA9890.1136697990032917No Hit
CGAGTCTCTGCTACGC9890.1136697990032917No Hit
GTAATCTCTACACAAA9810.11275032641276962No Hit
CTACAACGAATCTGTG9760.11217565604369334No Hit
CATTCTATGCCTAGCG9650.11091138123172548No Hit
TTGCGGTCTTTTGGGT9530.10953217234594237No Hit
GGCTGACTGAAACTGA9450.10861269975542029No Hit
CTAGCTCACGAATAGT9360.10757829309108295No Hit
TCCGGGTGAACACCTA9350.1074633590172677No Hit
TCGGACCGATAGGCGA9280.10665882050056089No Hit
CTTTCGTCTCGAACGA9250.10631401827911512No Hit
TGCTAGTGAAGGCGTA9200.1057393479100388No Hit
ACCAAGTTGAAGCTAA9150.1051646775409625No Hit
ATGTCCAGACCGCTAG9080.1043601390242557No Hit
CGAGTCTCTTGGAACG9070.10424520495044041No Hit
AGCCAATTGTTATGGC9000.1034406664337336No Hit
ACGAGGATGAACTAGA8930.1026361279170268No Hit
TAGTACCGAACGAGCA8910.10240625976939627No Hit
TGTAAGCACGCGCTGT8900.10229132569558103No Hit
AGACTACTGGCCCTAG8880.1020614575479505No Hit
TGCGCAGACATCAGGG8850.10171665532650472No Hit
CATAGTCGAAGATTCC8790.10102705088361316No Hit
GGGCCTTGACTATCTT8770.10079718273598264No Hit
CACATTACTGATGGAC8760.10068224866216738No Hit
AAAGCCTTGAACCAAA8760.10068224866216738No Hit
AGGCGATGACCTGCGA8740.10045238051453685No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)