FastQCFastQC Report
Thu 1 Sep 2022
EGAF00004949982

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004949982
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences540727
Sequences flagged as poor quality0
Sequence length16
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATGAATGACTCCTAC34650.6408039546758346No Hit
CTCCTAGTGAACGTAA27330.5054306516966972No Hit
TAGCTTTACCCTATCC23450.4336754036695042No Hit
CGACCAGACGCGCTGT22840.4223942950879094No Hit
CCAGGACTGTAACTCG18820.3480499401731373No Hit
ATCTGTACTGGGACTA18030.33343997987894075No Hit
GTTCTCGACCGTAGAG17260.3191998919972555No Hit
CTTCCTTTGGCTCAGC16670.30828865582817205No Hit
CGATCAAACCCGGCTA15600.2885004817588173No Hit
GGGGGGGGGGGGGGGG13950.25798600772663466No Hit
TGCTGCTACTTTCTTG13590.2513283043014312No Hit
ACAGCCGACTTGCGTA13110.24245136640115994No Hit
GGCAACAGAATAGCAA11900.22007408544422602No Hit
AACTTACCTCACCTAA11560.21378625443153385No Hit
CGCGTTGGAGCGGTTC11130.20583399756254078No Hit
ATCGAAAGATTTCCGC10660.19714199586852518No Hit
GTAACTGTGTGAAAGT10310.19066922864957733No Hit
CCCTGAACTAGCTTTG10140.18752531314323123No Hit
CAATTCGGATAACGGG9880.18271697178058427No Hit
AGTGGAGTGAGTTGAA9800.18123748213053908No Hit
GGGTAGAACCAGTCTA9180.16977143734268862No Hit
AAGAACCCTTAACCCA8980.16607271321757558No Hit
TTTAGTCTGAGCAATA8810.1629287977112295No Hit
TTCCTAAACTAATGCC8770.1621890528862069No Hit
CGCGATCGATATCTGG8700.16089449944241735No Hit
GTGAAGCGATCCCAGG8510.15738071152355995No Hit
TGGAACACTCCTAGTA8210.1518326253358904No Hit
TCCGGGAACGTTCTCC8060.14905858224205562No Hit
TGATAGAGAGACTGTT7830.1448050494981756No Hit
CATAGGTACAAGGTGC7670.14184607019808518No Hit
AGTCCTGCTCGAACGA7640.1412912615793182No Hit
TACGTTTACTGCACCG7630.14110632537306256No Hit
TGCAGTATGACACCGC7620.1409213891668069No Hit
CTTTAACTGTCCGTAA7540.1394418995167617No Hit
GCAACTTGAAGGTGCA7540.1394418995167617No Hit
TGCAGATACTGCCACG7520.13907202710425037No Hit
AGGTGTGGATCATGTG7380.13648292021667124No Hit
CACATCCTGAGCCTAT7270.1344486219478591No Hit
TCTCAGAGATGCATGT7200.13315406850406952No Hit
CTATTGGGACTTGTGA7190.13296913229781387No Hit
ATTTCTGACGGGTAGT7180.1327841960915582No Hit
TCTATGGGATGCTCAA7150.13222938747279128No Hit
AGAGTACCTTAATCGC7130.13185951506027996No Hit
CGAGTCTCTGCTACGC6980.12908547196644515No Hit
TCAAAGCACTCTGCAA6970.12890053576018953No Hit
ATGTACGTGTACTTTC6830.12631142887261038No Hit
CTTAGATCTTGTCACA6690.12372232198503126No Hit
TGAACTGTGAACGTAA6670.12335244957251995No Hit
TCCATTTGAAATCTGC6650.12298257716000865No Hit
TGGCAATTGAGTGGTG6630.12261270474749734No Hit
GAGTGTTACACCTGCG6620.12242776854124168No Hit
GGTCGCATGGTACTAC6510.12039347027242953No Hit
TTCTTAGACGTCCCGA6490.12002359785991822No Hit
TGAGGGATGTACGTTG6480.11983866165366257No Hit
GGTCTAAACTCCTACC6480.11983866165366257No Hit
GGGTGTGCTCGCAAAG6310.11669474614731648No Hit
CTACAACGAATCTGTG6210.11484538408475997No Hit
CTCAATTTGGAATCTC6200.11466044787850431No Hit
TCGGACCGATAGGCGA6170.11410563925973735No Hit
CACCAGGTGCGCCACA6140.1135508306409704No Hit
GGGATGTGACTGGAAG6110.11299602202220344No Hit
TACTGTTTGCTTAAGC6100.11281108581594779No Hit
TTGCGGTCTTTTGGGT6080.11244121340343648No Hit
ATGTCCAGACCGCTAG5940.10985210651585735No Hit
CTTTCGTCTCGAACGA5930.1096671703096017No Hit
GGCTGACTGAAACTGA5920.10948223410334604No Hit
CTAGCTCACGAATAGT5870.1085575530720678No Hit
TGCGCAGACATCAGGG5830.10781780824704519No Hit
CGAGTCTCTTGGAACG5820.10763287204078953No Hit
ACCAAGTTGAAGCTAA5790.10707806342202257No Hit
TAGTACCGAACGAGCA5790.10707806342202257No Hit
GCATGATACTTGCGTA5760.10652325480325561No Hit
AGACTACTGGCCCTAG5730.10596844618448865No Hit
CACATTACTGATGGAC5730.10596844618448865No Hit
CATTCTATGCCTAGCG5700.10541363756572171No Hit
AGCCAATTGTTATGGC5660.1046738927406991No Hit
GTAATCTCTACACAAA5650.10448895653444344No Hit
AGGCGATGACCTGCGA5590.10337933929690953No Hit
TGTAAGCACGCGCTGT5570.10300946688439822No Hit
TGCTAGTGAAGGCGTA5560.10282453067814257No Hit
TCCGGGTGAACACCTA5550.10263959447188692No Hit
AGCTTGTGATTGCGTA5530.10226972205937561No Hit
GTCTAATCTAAGTTCC5520.10208478585311997No Hit
TCATCATTGGACTCGC5490.10152997723435302No Hit
CATAGTCGAAGATTCC5480.10134504102809737No Hit
ACTCAGAACGGAATTA5470.1011601048218417No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)