FastQCFastQC Report
Sun 4 Sep 2022
EGAF00004949992

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004949992
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12728676
Sequences flagged as poor quality0
Sequence length8
%GC62

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGAGCA1243159597.66604947757331No Hit
GTGGCGCA694710.5457833949108297No Hit
GTGGAGCC325010.25533684728875183No Hit
GTGGAGCT240720.1891162914351815No Hit
GAGGAGCA163240.1282458599778956No Hit
GTGTAGCA159700.12546473804502528No Hit
GTGGACCA150220.11801698778411832ABI Solid3 EF1 alpha Antisense Primer (100% over 8bp)
GTGGTGCA141080.11083635092919326No Hit
GTGGAGAA134580.10572977110895115No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)