FastQCFastQC Report
Mon 12 Sep 2022
EGAF00004949995

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004949995
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17015608
Sequences flagged as poor quality0
Sequence length16
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCACTGTCTCTTTGTT1820091.0696591035712624No Hit
TGCTGCTACCGTTCTC1755951.0319643000708525No Hit
AAACGACCTCTTCAAG1693110.9950335010068404No Hit
ATGGGTTACAATCGAA1659400.9752222782753341No Hit
TATGCTTTGGACTGTT1635960.9614466905913676No Hit
AAAGCGGACTCGGGCT1526840.897317333591606No Hit
GCAGGTCTGGCTGTTA1516980.8915226537893916No Hit
TCCAAAGTGCTCACGG1445900.84974924199006No Hit
TAGTGCATGAACCCGC1355800.7967978575905134No Hit
GAAGTATGAACAGGTG1351370.794194365549559No Hit
TGTGGCTCTTACGATC1282540.7537432691209154No Hit
TAACTCACTGGTGTCC1261890.7416073524966019No Hit
TTCCCATCTCTTTGTT1160560.6820561451580219No Hit
GCCTCTTGATCTGTGA1134320.6666350094571994No Hit
TCACCTGACTCAATGC1105700.6498151579420494No Hit
GGAACTACTACCAAGG1072060.6300450739109645No Hit
TGAGAATGAGCAGTTT1067780.6275297362280561No Hit
GAAGTAATGAGTCTGT1020400.5996847129999704No Hit
ATGTCCAGAGTCGGTC1017740.5981214423839571No Hit
CTAGAGACTAATCTGC1010620.5939370488553802No Hit
TTCGTCCGACCTGTGG993600.5839344676957767No Hit
TCGTATACTGCTAATC992190.5831058167301456No Hit
TCACACAGATTGGGAG975380.5732266516718062No Hit
TGCGGTGACCTTAGTG969360.5696887234355658No Hit
ATTCCTAACTGCTACA954750.561102488961899No Hit
CCATGATGATTCGGGC948740.5575704376828615No Hit
TCCGCGAGAGAACAGC942770.5540618942326363No Hit
AGGTCGAGAAGGGAGG937400.5509059682145945No Hit
ACTGATTCTCGGCTCA924670.5434246016951025No Hit
TGGACCTCTAGGGAGG897780.5276214637760813No Hit
GCAATCATGGAACGTG877640.5157852719691239No Hit
TGGCCTGGACCCGAGT851820.5006109684708299No Hit
TTCGATGGAAATCCTG792020.465466764396547No Hit
ACGAGGATGGAGCTGA779740.45824986095119263No Hit
AAGGTGCGAACAGATA757570.4452206468320145No Hit
TTTCGTAACAGTACGG743720.4370810611057801No Hit
TGGTCCTTGGAGAGGG719300.42272953161591403No Hit
TCCCAGACTGTCTTGA715780.4206608426804379No Hit
TCTCTCTACTCTGGAT702350.4127680891567319No Hit
GCGGTTCGAGTACGTT697860.41012933537255913No Hit
TCGCCGAGAAGGTAAT630580.3705891673103894No Hit
TTCGATGGACAGGTGA623620.36649880509706145No Hit
TGATATCCTCGATGTT622930.3660932950500505No Hit
TCCTTCACTTGTCACA621560.36528815191323166No Hit
AGCTGCTCTTGTCACA619180.363889436098904No Hit
AACTCCCTGCTTCACC601690.35361063795075676No Hit
CTTTCGGTGCTTTGTT600190.35272909437029815No Hit
GGGGGGGGGGGGGGGG567640.3335995986743465No Hit
AGCCCAGTGAAAGTAC567090.33327636602817834No Hit
TCGGCTTACCTCCTAC552030.324425668480374No Hit
CCCTTGCCTCCTACGG547090.3215224516220637No Hit
AACCCTCACGCGGTTC541230.3180785547010721No Hit
ATGGCCGTGATACGCT540170.317455597237548No Hit
TGTTTGTTGCAAGTTC528790.3107676199404688No Hit
AAACACGCTGCAGTTT524590.3082992979151847No Hit
CTAACTGGAACCTTGA497440.29234335910888404No Hit
TACAACGTGAGCAGGT490140.2880531803506522No Hit
CAATGTGTGGTTGACT487810.2866838493223398No Hit
TGGCCTTTGCGAAAGC481840.2831753058721146No Hit
AAATAGGACTCTATCT477750.2807716303760641No Hit
ACAGCCGACAAACATG469860.27613471114285193No Hit
TCAAAGGCTCTTAGTG463760.27254976724898694No Hit
CTTTCGGTGAGCCATT458380.26938796427374206No Hit
GGACATTTGGCCGCTA455730.2678305706149319No Hit
ACCCTTGTGGGCCATC455440.26766013885604323No Hit
TGAACTGTGTGCTGGC446340.2623121078012611No Hit
AGTGTTCGATTCGCCT423020.24860704360373134No Hit
CAGCCGATGTAATAGG419440.2465030929250368No Hit
GAGGTTGGAGGAATAT415630.244263972230672No Hit
GATAAGAACATTGGTC390920.2297420109819173No Hit
GGCTTGCGAATACGCT381720.22433521035510454No Hit
GTGCTAATGGGAGTCA375890.2209089443057221No Hit
CGACGTTCTTGCTACA369880.21737689302668467No Hit
GTAAAGCCTGAAATCA363660.21372142564638302No Hit
TTGGGAGGAATCTGTG359060.21101802533297664No Hit
TGTACTGGAAATGCTC347910.20446521805156773No Hit
GGAACATCTTTACGAC336240.1976068089955998No Hit
AGGGACCCTAATCCTG326090.1916416974344966No Hit
GGGTGAAACCGACTAT324790.19087769299809915No Hit
TAACGAGCTTACTTTC317750.18674031512714678No Hit
CGGATGAGAACAGGCA316100.18577061718864232No Hit
TCTTGAACTCGTACAT315480.18540624584205279No Hit
TACGTGTGAAACGCAG315190.1852358140831641No Hit
TACCACCTGAAACATG309540.18191533326343673No Hit
AGCTTGTGAACCGTGC307280.18058714093554576No Hit
ACTTATCTGTCAGGTT300290.1764791478506087No Hit
CATGATGTGCCTAGTA299540.1760383760603794No Hit
AACTAGCACTGCCACG285920.1680339603498153No Hit
ACGTGTTGATCTTTGT271200.1593830793469149No Hit
TGGTAGGGATGTGCTA263940.1551164084174953No Hit
GTAGATAGAGCTGAGA262720.15439941963872228No Hit
AACGAACCTGACACCC261930.15393514001968076No Hit
CGTCGTACTTGCGCCA261900.1539175091480716No Hit
AACTACGACATTGCCA261460.15365892303113707No Hit
TCGGACCGAATGTCCA258720.15204863675749936No Hit
GGTAGTTTGTCCAGCG253800.14915717381359514No Hit
CTCGGATCTAGTACGG253330.14888095682505142No Hit
AGTTTGCGAGCAGGAT249000.1463362343561276No Hit
TTTAGGGACTGGGCAA246680.14497278028501834No Hit
TACCACCTGTACTTTC246460.14484348722655108No Hit
CCTTCAGTGCAACGCC244840.14389142015965578No Hit
TGAGGAGTGGTCCCAG239600.14081189458525373No Hit
CTTTAACTGTTCGCCT235700.13851988127606135No Hit
AGAACGCGACACAGTT223620.13142051697476811No Hit
GAGAAGAGATGCTACA221860.13038617250703002No Hit
GAAAGTCCTTATCTGG217350.12773566480845114No Hit
CTTACATTGGAAGGCT217290.1277004030652328No Hit
GCCATAATGACCGTTA215830.12684236731358645No Hit
GGGATGTGATGCATGT214890.12628993333649907No Hit
CAAAGACCTGCTTTGA214250.1259138080755034No Hit
CGATCGGTGCGAGGAT212520.12489709447937446No Hit
CAGGCCATGTCGTAGT210360.12362767172351408No Hit
AATTCTGCTTAGCGAG206720.12148845930160122No Hit
TGCAATGACTCCGTAA205540.12079497835164044No Hit
GCTGGACACATTGGTC205530.12078910139443738No Hit
AACTACGACGCTGTTA203000.11930223122206389No Hit
CCGTTACTGTTGGTTG202330.11890847508945904No Hit
GGACCACTGGCTAGAG201060.11816210152467077No Hit
GAAGGACACCCAGCTG200780.11799754672298515No Hit
TCCTCATCTTTCTGGA197530.11608753563199152No Hit
GGCTTAAACTGCATCA192550.11316081094486898No Hit
ATGGCCGTGGGCCAAA188290.11065722717636656No Hit
TGTCTTCCTGAAATCA188260.11063959630475738No Hit
GCGTTTAGAAGTCTAC188190.11059845760433597No Hit
CCCACCTCTTGACCTA186900.10984033012514158No Hit
TCGTAGAACCGCACTG185670.10911746438916552No Hit
ATAGCCAACATCTGTG184790.10860029215529647No Hit
ACAAGACCTAAGCTAA181490.10666089627828755No Hit
CCTGAGGACTGACTCA180390.10601443098595124No Hit
AGGTGATCTGTTAGTC179360.10540910439403636No Hit
TATGGGTACCCTTTGC178070.10465097691484196No Hit
TCCGTAAGATTACGCA178000.10460983821442055No Hit
GCGTTAATGGTACGTT170250.10005519638205111No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)