FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004950000

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004950000
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17015608
Sequences flagged as poor quality0
Sequence length34
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCCGTTA173450.10193582268702946No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGTTA21350.024.33916728
GACCCGT24850.021.02229926
ACCCGTT25700.020.5996127
ACGAGAC27600.019.22813822
TCTCCGA28050.019.11288311
ATCTCCG28850.018.72844710
TCCGAGC29150.018.72783913
AGACCCG28100.018.6405725
CCCACGA29750.017.78734219
CCACGAG30750.017.4371420
CACGAGA30950.017.28049521
CGAGCCC32150.016.76264815
CTCCGAG33950.016.16242812
GCCCACG34600.015.77913918
CCGAGCC36300.015.231873514
CTTATAC44550.013.7014961
AGCGTCA10850.012.9014033
TATACAC67300.012.4172663
ACATCTC53150.011.6143698
TACACAT66800.010.9803235
Produced by FastQC (version 0.11.5)