FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004950009

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004950009
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17202468
Sequences flagged as poor quality0
Sequence length34
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG174080.10119478205104057No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACTG21100.09.0240845
ATACCGT5150.08.9713646
GTACTAG10950.08.8235281
TATACTG17700.08.7800395
TACACTG29500.08.637665
TACACCG9950.08.44257455
TAGACAG27850.08.3953345
GTGTTAG28000.07.9014421
CCTATAC13400.07.8354513
TGTACTG23950.07.77486475
CTTACAC22150.07.77393
GTGTAGG26850.07.77050641
CTAGGAC24150.07.76774933
GTCTAGG22100.07.72991751
TAGGACC17400.07.72431334
ACTGTCC28650.07.72157148
GTCCTAG23400.07.71935751
CTAGACT20200.07.69324354
CCTACAC26000.07.64579343
GTAAGAC19500.07.6098973
Produced by FastQC (version 0.11.5)