FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004950011

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004950011
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5375299
Sequences flagged as poor quality0
Sequence length8
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTAGCTGT523950297.47368471967792No Hit
CTAGCTGA301470.5608432200701766No Hit
CTAGCAGT102030.1898126969309056No Hit
CAAGCTGT88820.16523731982165085No Hit
CTAGCTAT84110.15647501655256757RNA PCR Primer, Index 38 (100% over 8bp)
CTAGATGT82310.15312636562170773No Hit
CTAGCTCT71270.13258797324576735No Hit
CTCGCTGT70960.13201126114100817No Hit
CTACCTGT69190.12871842105899597No Hit
ATAGCTGT63790.11867246826641645No Hit
CTAGCTGG59000.10976133606707274No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)