FastQCFastQC Report
Sun 4 Sep 2022
EGAF00004950025

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004950025
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3127711
Sequences flagged as poor quality0
Sequence length16
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGCGGTGAGTCTTGA88520.2830184758118637No Hit
GGGGGGGGGGGGGGGG79680.2547549949467838No Hit
GGACACCTGGCTCCTT73400.23467641351774507No Hit
GTTAAGAGAGTACGTT59440.19004313378058266No Hit
CGCTTACTGATCACAC55440.17725422841176822No Hit
TTGTGGTGAAAGACAT52130.16667140921907425No Hit
ATCTTACTGGTTTGGT51950.16609590847747763No Hit
TACCAGTGATACGATC51690.1652646296285047No Hit
ATAGATGGAGAGTAAT49980.1597973725833365No Hit
ATTCCTAACTGCGTGC49440.15807087035854656No Hit
ATATGCCGAGAACAGC45970.14697649495110002No Hit
TGGAACACTTGAGGCA44020.14074190358380298No Hit
CTTGACGCTCAGGGTA43150.13796031666608582No Hit
AGCTCTGCTGGCCTGT40810.13047880702532938No Hit
CTTTCTGCTCTTGTGA40600.12980738949346662No Hit
NNNNNNNNNNNNNNNN39470.12619452372677656No Hit
GCGGTTCGAAGCAAGC39470.12619452372677656No Hit
ACTGGAACTCTGGAAG37490.1198640155692134No Hit
TGTGACTGACCGTTAA36480.11663481696358774No Hit
AGGTAGCGATTGACCA36160.11561170453408258No Hit
AGTTCACGACCAGAGA35630.11391717457271468No Hit
TGTACCCTGCTGAGAG35450.11334167383111804No Hit
ACGCTCTTGCCTCAAA35390.11314984025058582No Hit
ACCCTTTCTAGGTGAC35240.11267025629925528No Hit
TCCCAGACTCCTAACA34780.1111995321818416No Hit
ACATGTGTGTCAATGC33800.10806625036648206No Hit
ACACTCCACCGTACAT33690.10771455546883967No Hit
TCAGTCCACCCAGCAC33500.10710708246382099No Hit
TTCCAAACTCCGCTAG33480.10704313793697691No Hit
CACAGCTCTCTCTGGG33460.10697919341013284No Hit
GCTATACGACAGGTGA33230.10624383135142601No Hit
CAGTGGAGAGATCATC31760.10154390862838669No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)