FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004950062

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004950062
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4488548
Sequences flagged as poor quality0
Sequence length8
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATTGGTG438600497.71543046882867No Hit
TAATGGTG113080.2519300228047021No Hit
TATTGGTA101330.22575229227803734No Hit
TATTGGGG92730.20659242142447848No Hit
AATTGGTG85240.1899055106462045No Hit
TATTGGTC74310.165554651526507No Hit
TATTGGAG66470.1480879785623324No Hit
TATTGGTT64420.1435208000449143No Hit
TATTGCTG60210.1341413748945093No Hit
TATTCGTG59430.1324036191659307No Hit
TATAGGTG57040.12707895738220912RNA PCR Primer, Index 39 (100% over 8bp)
TTTTGGTG48540.10814187572462186RNA PCR Primer, Index 28 (100% over 8bp)

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)