FastQCFastQC Report
Sun 4 Sep 2022
EGAF00004950063

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004950063
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4061368
Sequences flagged as poor quality0
Sequence length16
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCTGCTACCGTTCTC405530.9985059221424899No Hit
AAACGACCTCTTCAAG399100.982673818279949No Hit
GCACTGTCTCTTTGTT397150.9778724804056169No Hit
ATGGGTTACAATCGAA383380.9439676483391803No Hit
TATGCTTTGGACTGTT375240.9239251404945329No Hit
AAAGCGGACTCGGGCT353660.8707903346852588No Hit
GCCTCTTGATCTGTGA331950.8173354396843625No Hit
TCCAAAGTGCTCACGG331340.8158334827082895No Hit
GCAGGTCTGGCTGTTA325510.8014787135763122No Hit
TAGTGCATGAACCCGC319880.7876163893545229No Hit
TAACTCACTGGTGTCC305070.7511508437551091No Hit
TGTGGCTCTTACGATC301590.7425823023178397No Hit
GAAGTATGAACAGGTG279510.6882163842330958No Hit
TTCCCATCTCTTTGTT268160.6602701355799326No Hit
TCACCTGACTCAATGC255230.628433572136285No Hit
TGAGAATGAGCAGTTT246980.6081202195910343No Hit
ATGTCCAGAGTCGGTC246050.6058303507586607No Hit
GGAACTACTACCAAGG241810.5953905186626772No Hit
CTAGAGACTAATCTGC237360.5844336194109965No Hit
TCGTATACTGCTAATC236370.5819960171055665No Hit
TTCGTCCGACCTGTGG236060.5812327274947752No Hit
GAGCCTGACCGAGTTT229590.5653021346501967No Hit
TGCGGTGACCTTAGTG228500.5626183098896727No Hit
TCACACAGATTGGGAG226400.5574476383326997No Hit
TCCGCGAGAGAACAGC223570.5504795428535411No Hit
ATTCCTAACTGCTACA223480.5502579426439563No Hit
ACTGATTCTCGGCTCA220310.5424526908174783No Hit
GAAGTAATGAGTCTGT217330.5351152616556786No Hit
AGGTCGAGAAGGGAGG216910.5340811273442839No Hit
TGGCCTGGACCCGAGT214860.529033567014858No Hit
TGGACCTCTAGGGAGG209630.5161561326134445No Hit
CCATGATGATTCGGGC204440.5033771871940685No Hit
ACGAGGATGGAGCTGA184870.4551914527321828No Hit
GCAATCATGGAACGTG182990.45056247057641663No Hit
TTCGATGGAAATCCTG178970.4406643278816399No Hit
AAGGTGCGAACAGATA176630.4349027224324415No Hit
TCCCAGACTGTCTTGA172190.42397044542627016No Hit
TTTCGTAACAGTACGG171970.42342875602506347No Hit
TGGTCCTTGGAGAGGG165540.4075966521625226No Hit
TCTCTCTACTCTGGAT165420.40730118521640984No Hit
TCGCCGAGAAGGTAAT147550.36330123249112123No Hit
TCCTTCACTTGTCACA145850.3591154507545241No Hit
TTCGATGGACAGGTGA144640.3561361590478873No Hit
TGATATCCTCGATGTT142310.3503991758441983No Hit
AGCCCAGTGAAAGTAC141510.3484293962034467No Hit
AGCTGCTCTTGTCACA140980.3471244171914488No Hit
AACTCCCTGCTTCACC140930.34700130596390183No Hit
CTTTCGGTGCTTTGTT138640.3413628117422504No Hit
AACCCTCACGCGGTTC136570.33626600692180564No Hit
GCGGTTCGAGTACGTT134620.3314646690474736No Hit
CCCTTGCCTCCTACGG133320.3282637771312523No Hit
ATGGCCGTGATACGCT132270.32567844135276586No Hit
TCGGCTTACCTCCTAC126830.31228393979565505No Hit
TGTTTGTTGCAAGTTC122460.3015240185080495No Hit
GGGGGGGGGGGGGGGG119670.29465441201092835No Hit
AAACACGCTGCAGTTT119100.29325094401689283No Hit
TGGCCTTTGCGAAAGC116640.28719387162158166No Hit
ACCCTTGTGGGCCATC115940.285470314435924No Hit
AAATAGGACTCTATCT115840.2852240919808301No Hit
CTAACTGGAACCTTGA114100.2809398212621954No Hit
TACAACGTGAGCAGGT113380.279167019585519No Hit
CAATGTGTGGTTGACT112110.27603999440582583No Hit
TCAAAGGCTCTTAGTG108910.26816087584281945No Hit
ACAGCCGACAAACATG108100.26616647395655846No Hit
CTTTCGGTGAGCCATT105240.25912451174087153No Hit
TGAACTGTGTGCTGGC102400.2521317940162034No Hit
AGTGTTCGATTCGCCT98320.2420859178483703No Hit
CAGCCGATGTAATAGG95800.2358811119800028No Hit
GGACATTTGGCCGCTA91750.2259091025486979No Hit
GAGGTTGGAGGAATAT85590.21074179931491063No Hit
CGACGTTCTTGCTACA83930.2066545065603511No Hit
TTGGGAGGAATCTGTG83530.2056696167399753No Hit
TGTACTGGAAATGCTC82180.202345613596207No Hit
GTGCTAATGGGAGTCA79760.19638703018293344No Hit
GGAACATCTTTACGAC76600.18860640060196465No Hit
AGGGACCCTAATCCTG76060.18727679934445735No Hit
TCTTGAACTCGTACAT75850.18675973218876005No Hit
TAACGAGCTTACTTTC74880.18437137437434875No Hit
CGGATGAGAACAGGCA73530.18104737123058043No Hit
TACCACCTGAAACATG73450.1808503932665053No Hit
GGGTGAAACCGACTAT72980.1796931477275637No Hit
TACGTGTGAAACGCAG72480.17846203545209396No Hit
GGACCACTGGCTAGAG72320.17806807952394366No Hit
AGCTTGTGAACCGTGC69820.17191251814659494No Hit
CATGATGTGCCTAGTA69230.17045980566154065No Hit
ACTTATCTGTCAGGTT67030.1650429116494738No Hit
ACGTGTTGATCTTTGT66570.16391028835604163No Hit
TCGGACCGAATGTCCA64760.15945366191884114No Hit
TGGTAGGGATGTGCTA63820.15713917084095802No Hit
AACTAGCACTGCCACG63240.15571108060141312No Hit
CTCGGATCTAGTACGG62410.15366743422413334No Hit
GTCCGAGTGGTCTTGA61810.15219009949356965No Hit
AACGAACCTGACACCC61310.15095898721809992No Hit
AACTACGACATTGCCA61170.15061427578096837No Hit
CGTCGTACTTGCGCCA60700.14945703024202683No Hit
GTAGATAGAGCTGAGA59210.145788315661127No Hit
CCTTCAGTGCAACGCC58760.14468031461320421No Hit
TACCACCTGTACTTTC58630.14436022542158208No Hit
TTTAGGGACTGGGCAA58360.14369542479282843No Hit
AGTTTGCGAGCAGGAT58180.14325222437365934No Hit
GGTAGTTTGTCCAGCG56910.14012519919396618No Hit
TGAGGAGTGGTCCCAG56670.1395342653017407No Hit
CTTTAACTGTTCGCCT55920.1376875968885361No Hit
GCCATAATGACCGTTA52730.1298331005710391No Hit
CGATCGGTGCGAGGAT51790.127518609493156No Hit
CTTACATTGGAAGGCT51660.12719852030153386No Hit
AGAACGCGACACAGTT51400.1265583419182896No Hit
CAAAGACCTGCTTTGA51090.12579505230749835No Hit
GTCCGTTACGGATGGT50730.12490865146916014No Hit
CCGTTACTGTTGGTTG50570.12451469554100981No Hit
GGGATGTGATGCATGT49000.12064900299603484No Hit
TGCAATGACTCCGTAA48930.12047664727746907No Hit
CAGGCCATGTCGTAGT48670.1198364688942248No Hit
AATTCTGCTTAGCGAG48170.11860535661875507No Hit
GGCTTGCGAATACGCT47440.11680793269656924No Hit
TCCTCATCTTTCTGGA47140.11606926533128739No Hit
AACTACGACGCTGTTA46810.11525673122947735No Hit
CCCACCTCTTGACCTA46520.1145426861097049No Hit
GAACCATACTTTGGGT45840.11286837341506606No Hit
GAGAAGAGATGCTACA44770.11023379314556081No Hit
ATGGCCGTGGGCCAAA44610.10983983721741049No Hit
GAAAGTCCTTATCTGG44040.10843636922337498No Hit
CCTGAGGACTGACTCA43870.10801779104971526No Hit
TGTCTTCCTGAAATCA43580.10730374592994281No Hit
TCGTAGAACCGCACTG43310.10663894530118916No Hit
AGGTGATCTGTTAGTC42290.10412747625923088No Hit
TATGGGTACCCTTTGC42250.10402898727719331No Hit
TCCGTAAGATTACGCA41920.10321645317538328No Hit
GAAGGACACCCAGCTG41450.1020592076364417No Hit
ACAAGACCTAAGCTAA40630.10004018350467134No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)