FastQCFastQC Report
Sun 4 Sep 2022
EGAF00004950072

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004950072
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3864528
Sequences flagged as poor quality0
Sequence length16
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCACTGTCTCTTTGTT412971.0686169177710707No Hit
AAACGACCTCTTCAAG381180.9863559016780316No Hit
TGCTGCTACCGTTCTC378760.9800938174079732No Hit
ATGGGTTACAATCGAA368800.9543209416518653No Hit
TATGCTTTGGACTGTT359400.9299971432475065No Hit
GCCTCTTGATCTGTGA340990.8823587253087569No Hit
AAAGCGGACTCGGGCT330480.8551626485821813No Hit
TCCAAAGTGCTCACGG320770.8300366823581042No Hit
GCAGGTCTGGCTGTTA297250.7691754335846447No Hit
TAGTGCATGAACCCGC296140.766303155262428No Hit
TAACTCACTGGTGTCC284500.7361830474510729No Hit
GAAGTATGAACAGGTG283510.7336212857042309No Hit
TGTGGCTCTTACGATC282470.7309301420509826No Hit
TTCCCATCTCTTTGTT253470.6558886363354076No Hit
TCACCTGACTCAATGC243770.6307885464926117No Hit
GGAACTACTACCAAGG238430.6169705588884334No Hit
GAGCCTGACCGAGTTT236580.6121834283514054No Hit
TGAGAATGAGCAGTTT234600.6070599048577213No Hit
CTAGAGACTAATCTGC224370.5805883668070202No Hit
GAAGTAATGAGTCTGT221650.5735499910985248No Hit
ATGTCCAGAGTCGGTC221530.57323947452315No Hit
TCGTATACTGCTAATC219250.5673396595910289No Hit
TCACACAGATTGGGAG218070.5642862465998435No Hit
ATTCCTAACTGCTACA215790.5583864316677224No Hit
TTCGTCCGACCTGTGG214610.555333018676537No Hit
TGCGGTGACCTTAGTG213590.5526936277858512No Hit
AGGTCGAGAAGGGAGG212850.5507787755710399No Hit
TCCGCGAGAGAACAGC207130.5359774854781748No Hit
ACTGATTCTCGGCTCA204370.5288356042445546No Hit
TGGACCTCTAGGGAGG202330.5235568224631831No Hit
TGGCCTGGACCCGAGT196200.5076946007377874No Hit
CCATGATGATTCGGGC193240.5000351918785425No Hit
GCAATCATGGAACGTG177690.45979741898622545No Hit
TTCGATGGAAATCCTG177330.4588658692601011No Hit
ACGAGGATGGAGCTGA175140.45319894175951114No Hit
TTTCGTAACAGTACGG168330.4355771261069916No Hit
AAGGTGCGAACAGATA167140.4324978367345249No Hit
TGGTCCTTGGAGAGGG163600.4233375977609685No Hit
TCCCAGACTGTCTTGA163290.42253542994125026No Hit
TCTCTCTACTCTGGAT157830.4084069257616971No Hit
GCGGTTCGAGTACGTT147810.3824787917179019No Hit
TCCTTCACTTGTCACA138740.35900891389582373No Hit
TTCGATGGACAGGTGA137950.3569646797746064No Hit
TGATATCCTCGATGTT137390.35551560242285735No Hit
TCGCCGAGAAGGTAAT136960.3544029180277643No Hit
AGCCCAGTGAAAGTAC133330.3450097916226768No Hit
CTTTCGGTGCTTTGTT130500.33768677572008793No Hit
AACTCCCTGCTTCACC130210.3369363606629322No Hit
AGCTGCTCTTGTCACA130050.33652233856243247No Hit
AACCCTCACGCGGTTC128820.3333395436648408No Hit
CCCTTGCCTCCTACGG125900.3257836403307208No Hit
ATGGCCGTGATACGCT125160.32386878811590963No Hit
TCGGCTTACCTCCTAC120350.31142224871963664No Hit
GGGGGGGGGGGGGGGG115870.2998296299056444No Hit
TGTTTGTTGCAAGTTC115700.2993897314238634No Hit
AAACACGCTGCAGTTT114610.296569205864209No Hit
ACCCTTGTGGGCCATC112270.29051413264440057No Hit
CTAACTGGAACCTTGA111420.28831464023549574No Hit
TGGCCTTTGCGAAAGC108490.28073286052009455No Hit
TACAACGTGAGCAGGT105030.271779632596788No Hit
TCAAAGGCTCTTAGTG104840.2712879813524446No Hit
CAATGTGTGGTTGACT104630.2707445773455387No Hit
AAATAGGACTCTATCT103760.26849333217407145No Hit
CTTTCGGTGAGCCATT99230.25677133145367304No Hit
TGAACTGTGTGCTGGC98870.2558397817275486No Hit
AGTGTTCGATTCGCCT92430.2391753921824347No Hit
CAGCCGATGTAATAGG90330.23374135211337582No Hit
GAGGTTGGAGGAATAT89810.2323957802867517No Hit
GGCTTGCGAATACGCT83160.2151879867347319No Hit
GATAAGAACATTGGTC82160.21260034860660862No Hit
ACAGCCGACAAACATG81300.21037497981642259No Hit
CGACGTTCTTGCTACA78490.20310371667639618No Hit
GTGCTAATGGGAGTCA78480.20307784029511494No Hit
TGTACTGGAAATGCTC78030.20191340313745948No Hit
TTGGGAGGAATCTGTG78010.201861650374897No Hit
GTAAAGCCTGAAATCA77180.1997139107285547No Hit
AGGGACCCTAATCCTG75020.19412461237180842No Hit
GGGTGAAACCGACTAT71370.18467973320415845No Hit
TCTTGAACTCGTACAT71060.18387756538444022No Hit
TAACGAGCTTACTTTC70880.18341179052137804No Hit
TACGTGTGAAACGCAG70620.18273900460806597No Hit
GGAACATCTTTACGAC69870.1807982760119735No Hit
TACCACCTGAAACATG68740.17787424492719422No Hit
GGACATTTGGCCGCTA68000.17595939271238298No Hit
GGACCACTGGCTAGAG67820.1754936178493208No Hit
CGGATGAGAACAGGCA67640.17502784298625862No Hit
AGCTTGTGAACCGTGC67580.1748725846985712No Hit
CATGATGTGCCTAGTA65770.17018895968666806No Hit
ACTTATCTGTCAGGTT64850.16780833260879466No Hit
AACTAGCACTGCCACG62590.16196027043923605No Hit
TCGGACCGAATGTCCA60460.15644860122633344No Hit
ACGTGTTGATCTTTGT60330.15611220826967745No Hit
TGGTAGGGATGTGCTA59470.15388683947949142No Hit
AACTACGACATTGCCA57960.14997950590602527No Hit
CGTCGTACTTGCGCCA57140.14785764264096415No Hit
CTCGGATCTAGTACGG56940.14734011501533953No Hit
GTAGATAGAGCTGAGA56550.14633093614537146No Hit
AACGAACCTGACACCC55530.1436915452546857No Hit
AGTTTGCGAGCAGGAT55360.14325164677290472No Hit
CCTTCAGTGCAACGCC55120.14263061362215515No Hit
TACCACCTGTACTTTC54910.14208720961524926No Hit
TTTAGGGACTGGGCAA54680.1414920528457809No Hit
GTCCGAGTGGTCTTGA54660.14144030008321845No Hit
GGTAGTTTGTCCAGCG53860.1393701895807198No Hit
TGAGGAGTGGTCCCAG53380.13812812327922064No Hit
CTTTAACTGTTCGCCT50430.13049459080125697No Hit
GCCATAATGACCGTTA49810.12889025516182054No Hit
CAGGCCATGTCGTAGT49610.12837272753619589No Hit
AGAACGCGACACAGTT48800.126276740652416No Hit
CTTACATTGGAAGGCT48150.1245947758691359No Hit
TGCAATGACTCCGTAA48080.12441364120016726No Hit
GGGATGTGATGCATGT47520.12296456384841824No Hit
GAGAAGAGATGCTACA47470.12283518194201207No Hit
GTCCGTTACGGATGGT46670.12076507143951344No Hit
GAAAGTCCTTATCTGG46560.12048043124541989No Hit
CCGTTACTGTTGGTTG45550.11786691673601538No Hit
GAACCATACTTTGGGT44910.11621082833401647No Hit
TCCTCATCTTTCTGGA44870.11610732280889155No Hit
AACTACGACGCTGTTA44730.11574505347095429No Hit
CGATCGGTGCGAGGAT44600.11540866051429825No Hit
AATTCTGCTTAGCGAG42700.11049214807086402No Hit
GCTGGACACATTGGTC42570.11015575511420798No Hit
GGCTTAAACTGCATCA42560.11012987873292676No Hit
TCGTAGAACCGCACTG42280.10940534005705226No Hit
TGTCTTCCTGAAATCA42050.1088101832875839No Hit
ATGGCCGTGGGCCAAA41650.10777512803633459No Hit
GAAGGACACCCAGCTG41470.10730935317327238No Hit
CCCACCTCTTGACCTA41460.10728347679199116No Hit
TCCGTAAGATTACGCA41440.1072317240294287No Hit
CAAAGACCTGCTTTGA41300.10686945469149144No Hit
GCGTTTAGAAGTCTAC41050.10622254515946061No Hit
CCTGAGGACTGACTCA41040.10619666877817939No Hit
ACAAGACCTAAGCTAA40990.10606728687177322No Hit
TATGGGTACCCTTTGC40680.105265119052055No Hit
ATAGCCAACATCTGTG39580.1024187171111194No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)