FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004950078

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004950078
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3628428
Sequences flagged as poor quality0
Sequence length8
%GC75

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGACAGGG344802895.02814993159572No Hit
GGCCAGGG663841.829552632710364ABI Solid3 GAPDH Forward Primer (100% over 8bp)
GGACAGGA414121.1413207041727162No Hit
GGACAGGC82030.22607586536097726No Hit
GGACACGG66790.18407420513787237No Hit
GGAAAGGG58660.16166780765664912No Hit
GGTCAGGG55610.15326196358312746No Hit
CGACAGGG55050.15171859549093988No Hit
GGACAGCG49140.13543055008946023No Hit
GCACAGGG44570.12283556405142944No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)