FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004950096

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004950096
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4670571
Sequences flagged as poor quality0
Sequence length8
%GC25

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGTTGAT455326697.48842272176142No Hit
AAGTTGAA203250.43517163104896595No Hit
AAGTTGCT123390.2641861134323833No Hit
AATTTGAT101940.21826025126264006No Hit
AACTTGAT95350.2041506274072271No Hit
AAGTTCAT94370.2020523828885162No Hit
AAGTAGAT81770.17507495336223344No Hit
AAGATGAT79010.16916561165647626No Hit
ATGTTGAT65220.13964031378604458No Hit
AAGTTTAT64230.1375206586089795No Hit
AAGTTGAG49000.10491222593554407No Hit
AAGGTGAT47850.1024500002248119No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)