FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004950100

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004950100
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2472351
Sequences flagged as poor quality0
Sequence length16
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCACTGTCTCTTTGTT275171.112989215528054No Hit
TGCTGCTACCGTTCTC252991.0232770346928894No Hit
AAACGACCTCTTCAAG245320.9922539315817211No Hit
ATGGGTTACAATCGAA237020.9586826465983187No Hit
TATGCTTTGGACTGTT232430.9401173215291843No Hit
GCCTCTTGATCTGTGA221270.8949780997924647No Hit
AAAGCGGACTCGGGCT217120.8781924573007635No Hit
TCCAAAGTGCTCACGG208330.8426392530834012No Hit
TAGTGCATGAACCCGC195020.7888038551160415No Hit
GCAGGTCTGGCTGTTA190350.7699149513964643No Hit
TGTGGCTCTTACGATC183650.7428152394219105No Hit
TAACTCACTGGTGTCC180320.7293462780972443No Hit
GAAGTATGAACAGGTG179200.7248161769910502No Hit
TTCCCATCTCTTTGTT168050.6797174025856361No Hit
GAGCCTGACCGAGTTT161250.6522132172980293No Hit
TCACCTGACTCAATGC158360.6405239385507964No Hit
GGAACTACTACCAAGG156640.6335669975662841No Hit
TGAGAATGAGCAGTTT154100.6232933754147368No Hit
ATGTCCAGAGTCGGTC146070.5908141683765776No Hit
CTAGAGACTAATCTGC145650.5891153804617548No Hit
TTCGTCCGACCTGTGG142040.5745138938605401No Hit
TCGTATACTGCTAATC141310.5715612386752529No Hit
TCACACAGATTGGGAG140180.5669906902377534No Hit
ATTCCTAACTGCTACA138820.5614898531802321No Hit
TCCGCGAGAGAACAGC136680.552834124280897No Hit
ACTGATTCTCGGCTCA135700.5488702858129771No Hit
AGGTCGAGAAGGGAGG135650.5486680491564507No Hit
TGGCCTGGACCCGAGT129720.5246827816924053No Hit
TGGACCTCTAGGGAGG129360.5232266777654143No Hit
CCATGATGATTCGGGC128260.5187774713218309No Hit
GCAATCATGGAACGTG124880.5051062733406381No Hit
TTCGATGGAAATCCTG111910.45264608463765865No Hit
ACGAGGATGGAGCTGA110890.4485204568445176No Hit
AAGGTGCGAACAGATA108960.44071412190259396No Hit
TTTCGTAACAGTACGG107620.4352941795076832No Hit
TCCCAGACTGTCTTGA106390.4303191577571307No Hit
TGCGGTGACCTTAGTG106100.42914618514927694No Hit
TGGTCCTTGGAGAGGG101680.4112684647123326No Hit
TCTCTCTACTCTGGAT101550.41074264940536354No Hit
GAAGTAATGAGTCTGT92020.3721963426714087No Hit
TCCTTCACTTGTCACA89890.36358106110337896No Hit
AGCTGCTCTTGTCACA89550.3622058518389986No Hit
TTCGATGGACAGGTGA89300.36119466855636595No Hit
TCGCCGAGAAGGTAAT89140.3605475112554811No Hit
AGCCCAGTGAAAGTAC89080.3603048272676493No Hit
TGATATCCTCGATGTT88510.35799932938324697No Hit
CTTTCGGTGCTTTGTT86440.349626731803049No Hit
AACTCCCTGCTTCACC86290.3490200218334694No Hit
CCCTTGCCTCCTACGG81750.33065693342086133No Hit
ATGGCCGTGATACGCT81210.32847277753037496No Hit
AACCCTCACGCGGTTC80480.3255201223450877No Hit
GGGGGGGGGGGGGGGG79060.3197766012997345No Hit
TCGGCTTACCTCCTAC77100.31184892436389494No Hit
AAACACGCTGCAGTTT75920.30707613926986904No Hit
TGTTTGTTGCAAGTTC75520.30545824601765686No Hit
ACCCTTGTGGGCCATC74150.2999169616288302No Hit
TGGCCTTTGCGAAAGC73920.2989866730088082No Hit
TACAACGTGAGCAGGT71930.2909376540790527No Hit
CTAACTGGAACCTTGA71910.29085675941644207No Hit
CAATGTGTGGTTGACT70630.2856795010093632No Hit
AAATAGGACTCTATCT67880.2745564849004045No Hit
GGACATTTGGCCGCTA67370.272493671003834No Hit
ACAGCCGACAAACATG67050.2711993564020643No Hit
TCAAAGGCTCTTAGTG66610.2694196738246309No Hit
CTTTCGGTGAGCCATT65230.2638379421044989No Hit
TGAACTGTGTGCTGGC64680.26161333888270716No Hit
AGTGTTCGATTCGCCT61360.24818482488934623No Hit
CAGCCGATGTAATAGG60430.24442322307795294No Hit
GGCTTGCGAATACGCT56990.23050934110892832No Hit
GATAAGAACATTGGTC55520.2245635834070486No Hit
GTGCTAATGGGAGTCA53190.21513935521291272No Hit
GTAAAGCCTGAAATCA51860.20975986014930728No Hit
TTGGGAGGAATCTGTG50690.2050275223865867No Hit
GGACCACTGGCTAGAG50300.20345007646567984No Hit
CGACGTTCTTGCTACA50180.2029647084900162No Hit
TGTACTGGAAATGCTC49910.20187263054477297No Hit
AGGGACCCTAATCCTG49840.20158949922563585No Hit
GGAACATCTTTACGAC49760.2012659205751934No Hit
GGGTGAAACCGACTAT46150.1866644339739786No Hit
TAACGAGCTTACTTTC45580.1843589360895763No Hit
CGGATGAGAACAGGCA45130.1825388061808376No Hit
TACGTGTGAAACGCAG44850.1814062809042891No Hit
TACCACCTGAAACATG44560.18023330829643525No Hit
TCTTGAACTCGTACAT44390.17954570366424508No Hit
ACTTATCTGTCAGGTT44310.17922212501380264No Hit
AGCTTGTGAACCGTGC43000.1739235246128078No Hit
CATGATGTGCCTAGTA42730.1728314466675646No Hit
AACTAGCACTGCCACG40430.16352856046734465No Hit
TGGTAGGGATGTGCTA39040.15790638141590735No Hit
ACGTGTTGATCTTTGT38750.15673340880805356No Hit
GTAGATAGAGCTGAGA38480.15564133086281032No Hit
CGTCGTACTTGCGCCA38080.15402343761059817No Hit
GTCCGAGTGGTCTTGA37780.15281001767143904No Hit
TCGGACCGAATGTCCA37500.15167749239489053No Hit
AACGAACCTGACACCC37390.15123257175053217No Hit
AACTACGACATTGCCA36750.14864394254699273No Hit
CTCGGATCTAGTACGG36560.14787544325219193No Hit
TTTAGGGACTGGGCAA36480.14755186460174952No Hit
TACCACCTGTACTTTC36170.14629799733128507No Hit
GGTAGTTTGTCCAGCG35920.14528681404865246No Hit
GCCATAATGACCGTTA35900.14520591938604188No Hit
TGAGGAGTGGTCCCAG35060.14180834355639632No Hit
CCTTCAGTGCAACGCC34750.1405544762859319No Hit
AGTTTGCGAGCAGGAT34750.1405544762859319No Hit
CTTTAACTGTTCGCCT32930.13319306198836653No Hit
GAGGTTGGAGGAATAT32300.13064488011613237No Hit
AGAACGCGACACAGTT31870.1289056448700043No Hit
CTTACATTGGAAGGCT31400.127004620298655No Hit
GTCCGTTACGGATGGT31160.1260338843473277No Hit
CAAAGACCTGCTTTGA31100.1257912003594959No Hit
GGGATGTGATGCATGT30910.1250227010646951No Hit
GAGAAGAGATGCTACA30780.12449688575772615No Hit
CAGGCCATGTCGTAGT30680.12409241244467312No Hit
CGATCGGTGCGAGGAT30600.12376883379423068No Hit
GAACCATACTTTGGGT30180.1220700458794079No Hit
CCGTTACTGTTGGTTG30070.12162512523504955No Hit
GAAAGTCCTTATCTGG29980.12126109925330182No Hit
TGCAATGACTCCGTAA29950.12113975725938589No Hit
AATTCTGCTTAGCGAG29710.1201690213080586No Hit
TCCTCATCTTTCTGGA28990.11725681345407671No Hit
AACTACGACGCTGTTA28960.1171354714601608No Hit
GGCTTAAACTGCATCA28600.11567936753316985No Hit
GCTGGACACATTGGTC28560.11551757820794863No Hit
CCCACCTCTTGACCTA27480.11114926642697578No Hit
CCTGAGGACTGACTCA27460.11106837176436517No Hit
TGTCTTCCTGAAATCA27340.11058300378870153No Hit
ATGGCCGTGGGCCAAA26900.10880332121126814No Hit
ACAAGACCTAAGCTAA26620.10767079593471962No Hit
TCGTAGAACCGCACTG26460.10702363863383477No Hit
TATGGGTACCCTTTGC26380.10670005998339233No Hit
ATAGCCAACATCTGTG26290.1063360340016446No Hit
AGGTGATCTGTTAGTC25770.10423277277376879No Hit
TCCGTAAGATTACGCA25500.10314069482852556No Hit
GGCTTCTACATCAGCT25300.10233174820241948No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)