FastQCFastQC Report
Sun 4 Sep 2022
EGAF00004950105

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004950105
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4017718
Sequences flagged as poor quality0
Sequence length16
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCACTGTCTCTTTGTT414241.0310330391530715No Hit
TGCTGCTACCGTTCTC385710.9600225799819698No Hit
AAACGACCTCTTCAAG385050.9583798564259612No Hit
ATGGGTTACAATCGAA365720.9102679680355864No Hit
TATGCTTTGGACTGTT357900.8908041828719687No Hit
GCCTCTTGATCTGTGA353960.8809976210376139No Hit
AAAGCGGACTCGGGCT335570.8352253692270089No Hit
TCCAAAGTGCTCACGG320350.7973431684354153No Hit
TAGTGCATGAACCCGC311410.7750917311767526No Hit
TAACTCACTGGTGTCC300530.7480116822534583No Hit
GCAGGTCTGGCTGTTA295110.7345214372935084No Hit
TGTGGCTCTTACGATC288430.7178950837266329No Hit
GAAGTATGAACAGGTG284060.7070182626057877No Hit
TTCCCATCTCTTTGTT255400.635684236673654No Hit
GAGCCTGACCGAGTTT248610.6187840958474438No Hit
GGAACTACTACCAAGG246340.6131341224048079No Hit
TCACCTGACTCAATGC244330.6081312824842361No Hit
ATGTCCAGAGTCGGTC238530.5936952269920388No Hit
CTAGAGACTAATCTGC236930.5897128668562601No Hit
TGAGAATGAGCAGTTT235330.5857305067204817No Hit
GAAGTAATGAGTCTGT229970.5723896002656235No Hit
TCGTATACTGCTAATC225640.5616123381481727No Hit
TTCGTCCGACCTGTGG221630.5516315480578776No Hit
TGCGGTGACCTTAGTG220240.54817187268992No Hit
TCACACAGATTGGGAG217190.5405804986810921No Hit
ATTCCTAACTGCTACA216190.5380915235962305No Hit
ACTGATTCTCGGCTCA209460.5213407212751119No Hit
TCCGCGAGAGAACAGC208550.5190757539478879No Hit
AGGTCGAGAAGGGAGG205640.5118328364509406No Hit
TGGCCTGGACCCGAGT205250.5108621361678446No Hit
TGGACCTCTAGGGAGG202960.5051623832235115No Hit
CCATGATGATTCGGGC191390.4763649414916627No Hit
GCAATCATGGAACGTG182280.4536903784685735No Hit
ACGAGGATGGAGCTGA179000.44552654019022736No Hit
TTCGATGGAAATCCTG176260.4387067484577066No Hit
AAGGTGCGAACAGATA171240.42621209353170136No Hit
TCCCAGACTGTCTTGA169800.4226279694095006No Hit
TTTCGTAACAGTACGG165340.4115271405310179No Hit
TGGTCCTTGGAGAGGG161170.401148114427145No Hit
TCTCTCTACTCTGGAT158200.39375585842510596No Hit
GCGGTTCGAGTACGTT157540.3921131348690973No Hit
TCCTTCACTTGTCACA145540.3622454338507581No Hit
TCGCCGAGAAGGTAAT140790.3504228021976654No Hit
AGCCCAGTGAAAGTAC138760.3453701827753964No Hit
TTCGATGGACAGGTGA137310.3417611689023471No Hit
TGATATCCTCGATGTT134830.3355885106918903No Hit
AACCCTCACGCGGTTC132160.3289429472153098No Hit
AGCTGCTCTTGTCACA131370.3269766568982691No Hit
AACTCCCTGCTTCACC131230.3266282003863885No Hit
CTTTCGGTGCTTTGTT129680.322770289004853No Hit
CCCTTGCCTCCTACGG128660.32023153441829416No Hit
ATGGCCGTGATACGCT125510.3123912629009801No Hit
TCGGCTTACCTCCTAC121380.3021117958005017No Hit
TGTTTGTTGCAAGTTC116790.29068740016098693No Hit
CTAACTGGAACCTTGA116190.28919401511007No Hit
ACCCTTGTGGGCCATC112770.28068172031984323No Hit
AAACACGCTGCAGTTT112060.27891454800959153No Hit
AAATAGGACTCTATCT111960.27866565050110537No Hit
TGGCCTTTGCGAAAGC111140.2766246909315188No Hit
TACAACGTGAGCAGGT108650.2704271429702134No Hit
TCAAAGGCTCTTAGTG107000.2663203340801918No Hit
GGGGGGGGGGGGGGGG106900.2660714365717056No Hit
CAATGTGTGGTTGACT105570.2627610997088397No Hit
CTTTCGGTGAGCCATT101120.25168516058120555No Hit
TGAACTGTGTGCTGGC97280.24212749625533697No Hit
AGTGTTCGATTCGCCT94840.2360543970482747No Hit
CAGCCGATGTAATAGG94290.23468546075160077No Hit
GAGGTTGGAGGAATAT93120.23177335990231268No Hit
ACAGCCGACAAACATG85200.2120606772302088No Hit
GATAAGAACATTGGTC85190.2120357874793602No Hit
GTGCTAATGGGAGTCA84640.2106668511826863No Hit
GGCTTGCGAATACGCT84170.20949703289280136No Hit
CGACGTTCTTGCTACA82550.20546489325532552No Hit
TTGGGAGGAATCTGTG82260.20474309048071565No Hit
GTAAAGCCTGAAATCA80050.19924245554317155No Hit
TGTACTGGAAATGCTC79670.19829664501092412No Hit
TCTTGAACTCGTACAT76130.18948567321051402No Hit
GGAACATCTTTACGAC75150.18704647762734966No Hit
AGGGACCCTAATCCTG74610.1857024310815244No Hit
GGGTGAAACCGACTAT73140.1820436377067778No Hit
TAACGAGCTTACTTTC72270.17987822938294823No Hit
TACGTGTGAAACGCAG69910.17400424818267485No Hit
CGGATGAGAACAGGCA69670.17340689416230806No Hit
TACCACCTGAAACATG69160.17213751686902865No Hit
GGACCACTGGCTAGAG69150.17211262711818No Hit
AACTAGCACTGCCACG68340.17009655729944212No Hit
CATGATGTGCCTAGTA66250.16489459937208137No Hit
TCGGACCGAATGTCCA65680.16347588357371023No Hit
AGCTTGTGAACCGTGC65660.16342610407201302No Hit
ACGTGTTGATCTTTGT65310.16255496279231146No Hit
GGACATTTGGCCGCTA65230.16235584478552254No Hit
ACTTATCTGTCAGGTT64300.16004109795660124No Hit
TGGTAGGGATGTGCTA62610.15583473006318513No Hit
AACTACGACATTGCCA61580.1532710857257777No Hit
CTCGGATCTAGTACGG60790.151304795408737No Hit
GTAGATAGAGCTGAGA60170.1497616308561228No Hit
CGTCGTACTTGCGCCA59920.1491393870849074No Hit
TTTAGGGACTGGGCAA58510.14562993221525256No Hit
AACGAACCTGACACCC58130.14468412168300512No Hit
GTCCGAGTGGTCTTGA57840.14396231890839525No Hit
GGTAGTTTGTCCAGCG57060.1420209183422032No Hit
TGAGGAGTGGTCCCAG55990.1393577150014013No Hit
TACCACCTGTACTTTC55960.13928304574885544No Hit
CCTTCAGTGCAACGCC55920.139183486745461No Hit
AGTTTGCGAGCAGGAT54850.13652028340465908No Hit
CTTTAACTGTTCGCCT52460.13057163295183982No Hit
CAGGCCATGTCGTAGT52270.13009872768571612No Hit
GCCATAATGACCGTTA52130.1297502711738355No Hit
GTCCGTTACGGATGGT50410.12546923402787352No Hit
CTTACATTGGAAGGCT50290.12517055701769014No Hit
AGAACGCGACACAGTT49730.12377673097016764No Hit
CGATCGGTGCGAGGAT49260.12260691268028269No Hit
TGCAATGACTCCGTAA49090.12218378691585621No Hit
GGGATGTGATGCATGT48360.12036683510390724No Hit
GAGAAGAGATGCTACA48130.11979437083438907No Hit
GAAAGTCCTTATCTGG48090.1196948118309946No Hit
GAACCATACTTTGGGT47830.11904767830893058No Hit
CCGTTACTGTTGGTTG47420.11802719852413734No Hit
CAAAGACCTGCTTTGA46380.11543866443588127No Hit
AACTACGACGCTGTTA46260.11513998742569787No Hit
TCCTCATCTTTCTGGA46170.11491597966806033No Hit
GCTGGACACATTGGTC45060.11215321732386395No Hit
GGCTTAAACTGCATCA45060.11215321732386395No Hit
AATTCTGCTTAGCGAG44590.110983399033979No Hit
GAAGGACACCCAGCTG44490.11073450152549281No Hit
CCTGAGGACTGACTCA43930.10934067547797033No Hit
ATGGCCGTGGGCCAAA43910.10929089597627309No Hit
TGTCTTCCTGAAATCA43570.10844464444742016No Hit
GCGTTTAGAAGTCTAC43400.10802151868299367No Hit
ACAAGACCTAAGCTAA43380.10797173918129645No Hit
TCCGTAAGATTACGCA43260.10767306217111307No Hit
CCCACCTCTTGACCTA42980.10697614914735182No Hit
TCGTAGAACCGCACTG41930.10436272530824711No Hit
ATAGCCAACATCTGTG41440.10314312751666495No Hit
GCGTTAATGGTACGTT40320.10035547542161993No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)