FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004950114

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004950114
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2953903
Sequences flagged as poor quality0
Sequence length8
%GC62

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCAAGGA286898297.12512563885815No Hit
GGCAAGGC122510.41473941425970995No Hit
GGCAAGAA92570.3133819898622263No Hit
GGCAACGA78720.26649487136172045No Hit
GGCAAGCA66110.22380558874140416Clontech Universal Primer Mix Long (100% over 8bp)
GCCAAGGA49440.16737177896498293RNA PCR Primer, Index 1 (100% over 8bp)
CGCAAGGA49260.1667624156920522No Hit
GGAAAGGA45390.1536611053240408No Hit
GGCAGGGA43530.14736435150375624No Hit
GGGAAGGA30470.10315166070111308No Hit
GGCAATGA30440.1030501001556246No Hit
GGCAAGTA29960.10142513142780923No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)