FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004950128

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004950128
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6622595
Sequences flagged as poor quality0
Sequence length8
%GC62

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCAGTCC640767796.75477663967071No Hit
CCCAGTCC564300.8520829070779657No Hit
ACCAGTCA442080.6675328930728816No Hit
ACCAGACC156190.2358441064265594No Hit
ACCACTCC144660.21843401264912016No Hit
ACAAGTCC121760.18385542223252366No Hit
ACCAGTAC82640.12478492192259984No Hit
ACCCGTCC78690.11882049257126549TruSeq Adapter, Index 16 (100% over 8bp)

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)