FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004950150

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004950150
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16130504
Sequences flagged as poor quality0
Sequence length34
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG211240.13095685044931019No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACCG7200.08.74949555
TACACTG25400.08.2672415
TATACTG17200.08.1390665
GTAGGAC14750.07.9695013
AGGACTG37800.07.92546945
GTATAGA13950.07.92359971
CTAGGAC22850.07.83912563
TAGGACA17500.07.83808764
TAGACTG19550.07.80517775
TACACAG37700.07.7608265
TAGGACT22850.07.71802954
GTATACG3651.4868419E-87.6666711
GTGTAAG21550.07.66133451
GTGTAGG26150.07.6512791
CGTATAC3854.9312803E-97.6331263
CCTACAC25150.07.62300973
GTATAGG11950.07.6105341
CTAACAC26300.07.50252063
GTATTAG15550.07.46822451
TAGACGG6950.07.4528085
Produced by FastQC (version 0.11.5)