FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004950153

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004950153
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2145196
Sequences flagged as poor quality0
Sequence length8
%GC62

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGAAGGG210039297.91142627526808No Hit
GAGAAGGA131820.6144893054061261No Hit
GTGAAGGG38120.17769938038295802No Hit
GAGCAGGG30230.14091952436980115No Hit
GAGAAGGC25930.12087473592156614TruSeq Adapter, Index 20 (100% over 8bp)
GAGAACGG23520.10964033123313673No Hit
GAGATGGG22580.1052584472467784No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)