FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004950155

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004950155
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2145196
Sequences flagged as poor quality0
Sequence length16
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACAGCCGACCAGGGAT377511.7597925783937693No Hit
GAAGCAGTGGAACCTT127780.5956565274222029No Hit
GTTCTTCCTAACGTAA112010.5221434311829781No Hit
GATACGTACCGCTCGA110960.5172487735386416No Hit
CGCTTAAGATAATAGG87510.4079347528151274No Hit
CATGGAGGATCGACAT85150.39693342706214263No Hit
TGTCCCATGAGACAAT84050.39180569048236147No Hit
AAAGCATGATCTTTGT77980.36350990771938785No Hit
TGAGCGCACAGGACAC76020.3543732134499598No Hit
TGTCGAAACTTCGCAA72700.3388967721364388No Hit
TAATTGCCTGTCTGGC69810.3254248096677413No Hit
GGGGGGGGGGGGGGGG67130.3129317787279111No Hit
GGAAGGACTAGGTAAT61930.2886915694416734No Hit
AATGATGGAAAGCTAA56910.2652904443230362No Hit
CATGTCAGAGGTTTGT56180.26188749186554516No Hit
TCGACCTTGTTTCGCC52590.24515242430062334No Hit
ATGGGTACTTGGCTGC44710.20841918407455542No Hit
GCTATGATGACCTGCG43460.20259221068844058No Hit
AAACGACCTAGGGAGG41870.19518030054130253No Hit
AGCCTACCTTAACTCG40370.18818793247796473No Hit
CAGCTCACTGTTTACG40250.1876285430328977No Hit
TTGCGATGACATTCAG39590.18455190108502906No Hit
TGTTCCTCTTTATGGC39040.18198803279513853No Hit
GTGAGATCTCGATTAC37060.17275810695153265No Hit
CAATGTGACCAACGCC36490.17010100708746428No Hit
CTGCCTTGACCATAAC36090.16823637560390753No Hit
TAACCAGACATCACAC35880.16725744407504023No Hit
CCTGTTGACCCTAGTA35560.16576573888819482No Hit
CACAGCTCTGGACTGA34890.1626424811532373No Hit
AGCTTGTGACTCACTC34630.16143047068892538No Hit
CATGCAAACGAAGGCT34240.15961245499245758No Hit
ATGGGTACTTTCGGGC33770.15742151299927837No Hit
CATGATGTGTCATGTG33540.15634934989623325No Hit
ATGCCGCGATGCCACG33290.1551839552190103No Hit
TTGTTTGTGATACGCT32520.15159453961316355No Hit
CTATTCAACCGAACGA32390.15098853438100762No Hit
TGGGCCATGCGTTCTC32160.14991637127796248No Hit
ATTAGCGTGGTCGGTC31470.1466998819688271No Hit
CATTAGTCTATTCTGG31380.14628033988502684No Hit
AGAACGCGAGGAATAT31110.145021713633626No Hit
GGGCTGACTAATACAG30800.14357662423386952No Hit
CACTATACTACGCCAC30700.14311046636298036No Hit
CCGCTATTGTTCGCAA30610.1426909242791801No Hit
GCAGATACTCGGCTCA29940.13956766654422254No Hit
GGGTCGTACCGCTCGA29430.13719026140268767No Hit
GCATCTCTGAGCCTAT29290.13653764038344282No Hit
GGCTTGCGAGTACGTT28850.1344865457515304No Hit
ATAGGAAACAGACTTC28670.13364746158392987No Hit
AGCTCGGACCTTAAGC28640.1335076142226631No Hit
CCCAATGCTGGAATGC28570.13318130371304066No Hit
AACCAACACCGGAATG28460.13266853005506257No Hit
TTGACGTGACCTCAAA28310.13196929324872878No Hit
GATACCATGTCTGGAT28090.13094374593277258No Hit
GGTAGCCTGTGCCACG28070.13085051435859474No Hit
AACGTCCCTTACAGAA27870.12991819861681636No Hit
CTGCCATTGCACAGTT27280.12716786717857015No Hit
CTGATTACTGCTTTGA27230.12693478824312557No Hit
GGGATCAGATGAGTAA27230.12693478824312557No Hit
TTGTAACCTCATCCTC27070.12618893564970288No Hit
ATAGCCAACATGGCCC27040.1260490882884361No Hit
TGGAGTTACAAGTCTC26730.12460399888867965No Hit
TGTTTGTTGCGCTCGA26300.12259952004385613No Hit
ATCTTACTGCGCACTG26300.12259952004385613No Hit
CGCTTACTGGCCACGT26140.12185366745043344No Hit
CGATCAAACCGATCGC25800.1202687306894102No Hit
CCTACGGGATGGAACG25400.11840409920585344No Hit
TGGGTGCACTTCTGAG25390.11835748341876454No Hit
CGCTGAGTGTTAGCTG25280.11784470976078643No Hit
CATGTTACTAACGTGG25160.1172853203157194No Hit
CGCTTCCCTGCGCTGT25070.11686577823191914No Hit
CTCGGATCTTTCTGAG24970.11639962036102995No Hit
TCAGGCTCTTAAAGCA24740.11532745725798482No Hit
TTCTAACACTACGTTG24370.11360267313569483No Hit
AGCCGGTACGCGCAAA24320.11336959420025024No Hit
CCTCCCTTGTTTCGCC24260.11308989947771673No Hit
TCCAAAGTGAAGGCCA24220.11290343632936103No Hit
GTCCTGACTTTACGAC24020.11197112058758268No Hit
ATCGCAATGTAACTCG23850.11117865220707104No Hit
TACAACCGATTCCATT23710.1105260311878262No Hit
AGTGGTACTCAACGCC23510.10959371544604782No Hit
CCTTCAGTGTAGCTTG23370.10894109442680296No Hit
TGAGATAGACTCATCC23220.10824185762046917No Hit
GCATAGTACGCTCCTT23180.1080553944721135No Hit
CTTAGGCCTTATGACC23130.1078223155366689No Hit
ATTGAGAGATGATCAC23020.1073095418786908No Hit
GCATGGGCTATGTGGG22950.10698323136906839No Hit
TATCTCCCTGGAATTA22790.10623737877564568No Hit
AACCACTGAAAACATG22720.10591106826602324No Hit
ACCTGCTTGCCAAACT22690.10577122090475648No Hit
GTAAGAAACGACAAAT22690.10577122090475648No Hit
GTGTACTGAATCACAC22530.1050253683113338No Hit
TTCTATGACCAACGCC22490.10483890516297813No Hit
AACAGCTACTCGAGAG22410.10446597886626675No Hit
CATGTCAGAACAGGTG22350.10418628414373325No Hit
AGAACAATGAGTCGGA22310.10399982099537758No Hit
CGAGTCTCTTACTTTC22300.10395320520828866No Hit
AGAACATGAAAGCTAA22240.10367351048575514No Hit
TACGCCTTGGGTTGAG22220.10358027891157731No Hit
TTCACGCTGTGGGCAA21800.10162241585384273No Hit
GTTGCTAGACGCTCGA21790.10157580006675382No Hit
GCATATCGAGCCGCTA21700.10115625798295354No Hit
GAAAGTAACCCTGCGA21660.10096979483459788No Hit
TGGTCCTTGTTTCTGT21590.10064348432497544No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)