FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004950160

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004950160
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1035962
Sequences flagged as poor quality0
Sequence length16
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATGAATGACTCCTAC56590.546255557636284No Hit
CTCCTAGTGAACGTAA52980.5114087196248511No Hit
CGACCAGACGCGCTGT50050.48312582893967154No Hit
TAGCTTTACCCTATCC47590.4593797842005788No Hit
CCAGGACTGTAACTCG36050.3479857369285746No Hit
ATCTGTACTGGGACTA34200.33012793905567966No Hit
GTTCTCGACCGTAGAG31870.3076367665995471No Hit
CTTCCTTTGGCTCAGC31530.3043547929364204No Hit
CGATCAAACCCGGCTA29790.2875588100721841No Hit
AGACCATTGAATCCAC28230.2725003426766619No Hit
TGCTGCTACTTTCTTG26920.25985509120990924No Hit
GGGGGGGGGGGGGGGG26310.2539668443437114No Hit
ACAGCCGACTTGCGTA23560.227421469127246No Hit
AACTTACCTCACCTAA22240.21467968902334256No Hit
CGCGTTGGAGCGGTTC20160.1946017324959796No Hit
CCCTGAACTAGCTTTG20130.19431214658452725No Hit
ATCGAAAGATTTCCGC20040.19344338885017018No Hit
GTAACTGTGTGAAAGT20000.19305727430156705No Hit
AGTGGAGTGAGTTGAA19690.19006488654989276No Hit
CAATTCGGATAACGGG18420.17780574963174325No Hit
TTCCTAAACTAATGCC17140.16545008407644296No Hit
GGGTAGAACCAGTCTA16900.16313339678482414No Hit
GGCAACAGAATAGCAA16750.1616854672275624No Hit
AAGAACCCTTAACCCA16740.1615889385904116No Hit
CGCGATCGATATCTGG16180.15618333490996775No Hit
TCCGGGAACGTTCTCC16170.15608680627281696No Hit
GTGAAGCGATCCCAGG15740.15193607487533328No Hit
TTTAGTCTGAGCAATA15530.14990897349516683No Hit
TGGAACACTCCTAGTA15270.14739922892924645No Hit
TGCAGATACTGCCACG15230.1470131143806433No Hit
CTTTAACTGTCCGTAA15160.14633741392058783No Hit
TCTCAGAGATGCATGT14520.14015958114293767No Hit
TGATAGAGAGACTGTT14300.13803595112562042No Hit
ATTTCTGACGGGTAGT14200.1370706647541126No Hit
CATAGGTACAAGGTGC14090.13600884974545396No Hit
TGCAGTATGACACCGC14090.13600884974545396No Hit
AGGTGTGGATCATGTG13970.13485050609964458No Hit
CACATCCTGAGCCTAT13870.13388521972813675No Hit
TCAAAGCACTCTGCAA13840.1335956338166844No Hit
CTATTGGGACTTGTGA13630.13156853243651795No Hit
AGTCCTGCTCGAACGA13480.1301206028792562No Hit
CGAGTCTCTGCTACGC13270.12809350149908974No Hit
AGAGTACCTTAATCGC13220.12761085831333582No Hit
TCTATGGGATGCTCAA13180.12722474376473267No Hit
CTTAGATCTTGTCACA12930.12481152783596308No Hit
TGGCAATTGAGTGGTG12910.12461847056166153No Hit
TACGTTTACTGCACCG12850.12403929873875683No Hit
ATGTACGTGTACTTTC12750.12307401236724899No Hit
TGAGGGATGTACGTTG12730.12288095509294743No Hit
GCAACTTGAAGGTGCA12500.12066079643847939No Hit
TGAACTGTGAACGTAA12440.1200816246155747No Hit
TCCATTTGAAATCTGC12430.11998509597842393No Hit
GGTCTAAACTCCTACC12210.11786146596110669No Hit
TACTGTTTGCTTAAGC12140.11718576550105118No Hit
TTCTTAGACGTCCCGA12130.11708923686390041No Hit
GAGTGTTACACCTGCG12100.11679965095244807No Hit
GGGTGTGCTCGCAAAG11780.113710734563623No Hit
CTCAATTTGGAATCTC11730.11322809137786907No Hit
AGCCAATTGTTATGGC11510.11110446136055184No Hit
GGTCGCATGGTACTAC11450.11052528953764712No Hit
GGGATGTGACTGGAAG11420.11023570362619478No Hit
TCCGGGTGAACACCTA11400.1100426463518932No Hit
CTTTCGTCTCGAACGA11310.10917388861753617No Hit
CTAGCTCACGAATAGT11250.10859471679463147No Hit
CGAGTCTCTTGGAACG11160.1077259590602744No Hit
TTGCGGTCTTTTGGGT11150.10762943042312363No Hit
CTACAACGAATCTGTG11150.10762943042312363No Hit
CACATTACTGATGGAC11080.10695372996306814No Hit
ATAGCCAACCGTTCTC11080.10695372996306814No Hit
GTAATCTCTACACAAA11020.10637455814016343No Hit
CACCAGGTGCGCCACA11010.10627802950301266No Hit
ACCAAGTTGAAGCTAA10940.10560232904295717No Hit
TGCTAGTGAAGGCGTA10930.1055058004058064No Hit
CTAACAGTGCATGATC10830.10454051403429857No Hit
CATTCTATGCCTAGCG10820.10444398539714778No Hit
CATAGTCGAAGATTCC10810.10434745675999699No Hit
TCGGACCGATAGGCGA10780.10405787084854465No Hit
ATGTCCAGACCGCTAG10660.10289952720273522No Hit
ACCTCAGCTAATCCGT10620.10251341265413211No Hit
TAGTACCGAACGAGCA10560.1019342408312274No Hit
TGTAAGCACGCGCTGT10560.1019342408312274No Hit
AGGCGATGACCTGCGA10540.10174118355692582No Hit
ACGAGGATGAACTAGA10490.1012585403711719No Hit
TCGTTGCTGTATTGCG10420.10058283991111643No Hit
AGACTACTGGCCCTAG10380.1001967253625133No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)