FastQCFastQC Report
Sun 4 Sep 2022
EGAF00004950167

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004950167
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3653938
Sequences flagged as poor quality0
Sequence length16
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACAGCCGACCAGGGAT667251.8261119920480315No Hit
GAAGCAGTGGAACCTT224960.6156645241380669No Hit
GTTCTTCCTAACGTAA189550.5187553811805236No Hit
CATGGAGGATCGACAT153700.42064205796595344No Hit
CGCTTAAGATAATAGG151770.4153600854749041No Hit
TGTCCCATGAGACAAT147900.40476877275969103No Hit
AAAGCATGATCTTTGT139670.382245128406667No Hit
GATACGTACCGCTCGA138450.3789062649667291No Hit
TGAGCGCACAGGACAC135200.37001175170459927No Hit
TAATTGCCTGTCTGGC128210.3508817062577416No Hit
TGTCGAAACTTCGCAA126550.3463386625607769No Hit
GGAAGGACTAGGTAAT107870.2952157371033663No Hit
GGGGGGGGGGGGGGGG106890.29253369925817024No Hit
AATGATGGAAAGCTAA98790.27036583543563136No Hit
CATGTCAGAGGTTTGT97590.2670817074619219No Hit
TCGACCTTGTTTCGCC94990.25996609685221805No Hit
GTGAGATCTCGATTAC80520.22036498703590482No Hit
ATGGGTACTTGGCTGC77300.21155257697311777No Hit
AAACGACCTAGGGAGG72610.19871711014253662No Hit
AGCCTACCTTAACTCG70200.19212148646200344No Hit
TTGCGATGACATTCAG68570.18766054596438145No Hit
TGTTCCTCTTTATGGC68400.1871952945014393No Hit
GCTATGATGACCTGCG66600.18226910254087508No Hit
CAGCTCACTGTTTACG65500.17925865189830809No Hit
CTGCCTTGACCATAAC64900.17761658791145335No Hit
CAATGTGACCAACGCC63000.1724167186197467No Hit
CCTGTTGACCCTAGTA62950.17227987995417546No Hit
TAACCAGACATCACAC62640.17143148022763385No Hit
CATGCAAACGAAGGCT61160.16738105572672554No Hit
CACAGCTCTGGACTGA60400.16530110801004286No Hit
CATGATGTGTCATGTG59490.16281064429664652No Hit
ATGCCGCGATGCCACG58560.16026544511702168No Hit
CTATTCAACCGAACGA58320.1596086195222798No Hit
ATGGGTACTTTCGGGC57570.1575560395387114No Hit
TGGGCCATGCGTTCTC56980.1559413432849709No Hit
AGAACGCGAGGAATAT56970.15591397555185665No Hit
ATTAGCGTGGTCGGTC56220.15386139556828823No Hit
TTGTTTGTGATACGCT55720.15249300891257594No Hit
CATTAGTCTATTCTGG55290.1513161963886634No Hit
CCGCTATTGTTCGCAA53930.14759418468512603No Hit
AGCTTGTGACTCACTC53930.14759418468512603No Hit
CACTATACTACGCCAC51680.1414364447344208No Hit
GGGCTGACTAATACAG50410.1379607426289116No Hit
AACCAACACCGGAATG50280.1376049620984264No Hit
TTGACGTGACCTCAAA50180.13733128476728396No Hit
ATAGGAAACAGACTTC50140.13722181383482698No Hit
AACGTCCCTTACAGAA49980.13678393010499906No Hit
AGCTCGGACCTTAAGC49960.13672919463877053No Hit
GGTAGCCTGTGCCACG48940.1339376858611175No Hit
CCCAATGCTGGAATGC48820.13360927306374656No Hit
GCAGATACTCGGCTCA48320.13224088640803427No Hit
CTGATTACTGCTTTGA48300.1321861509418058No Hit
CTGCCATTGCACAGTT47870.13100933841789325No Hit
CCTACGGGATGGAACG47370.12964095176218096No Hit
TGGAGTTACAAGTCTC47370.12964095176218096No Hit
TTGTAACCTCATCCTC47210.12920306803235304No Hit
TCAGGCTCTTAAAGCA46870.1282725651064687No Hit
TGTTTGTTGCGCTCGA46070.12608314645732904No Hit
ATAGCCAACATGGCCC46000.12589157232552933No Hit
GGGATCAGATGAGTAA45880.12556315952815839No Hit
GCATCTCTGAGCCTAT45450.12438634700424583No Hit
TCCAAAGTGAAGGCCA45210.12372952140950394No Hit
CGATCAAACCGATCGC44900.12288112168296232No Hit
CGCTTACTGGCCACGT44790.12258007661870562No Hit
CGCTTCCCTGCGCTGT44780.12255270888559139No Hit
CGCTGAGTGTTAGCTG44400.12151273502725006No Hit
TGGGTGCACTTCTGAG44220.12102011583119364No Hit
CATGTTACTAACGTGG43870.12006224517219503No Hit
TTCTAACACTACGTTG43750.1197338323748241No Hit
GATACCATGTCTGGAT43380.11872122624959701No Hit
ATCTTACTGCGCACTG43370.11869385851648276No Hit
CTCGGATCTTTCTGAG42720.1169149558640568No Hit
AGCCGGTACGCGCAAA42360.11592971747194397No Hit
CGAGTCTCTTACTTTC41910.1146981694818029No Hit
AGTGGTACTCAACGCC41550.11371293108969008No Hit
CTTAGGCCTTATGACC41450.11343925375854763No Hit
TACAACCGATTCCATT41190.11272769269757724No Hit
AACAGCTACTCGAGAG40760.11155088017366467No Hit
CCTCCCTTGTTTCGCC40710.11141404150809348No Hit
ACCTGCTTGCCAAACT40450.11070248044712308No Hit
ATTGAGAGATGATCAC40070.10966250658878175No Hit
TACGCCTTGGGTTGAG39920.10925199059206805No Hit
AGAACAATGAGTCGGA39840.10903304872715411No Hit
TATCTCCCTGGAATTA39710.1086772681966689No Hit
TGGTCCTTGTTTCTGT39660.10854042953109767No Hit
GTGTACTGAATCACAC39500.10810254580126974No Hit
AACCACTGAAAACATG39320.10760992660521333No Hit
ATCGCAATGTAACTCG39230.10736361700718514No Hit
GCATGGGCTATGTGGG39220.10733624927407087No Hit
ACCTCCATGGAATTGA38800.10618680448327257No Hit
CATGTCAGAACAGGTG38690.10588575941901587No Hit
TTCACGCTGTGGGCAA38690.10588575941901587No Hit
CCTTCAGTGTAGCTTG38640.10574892075344465No Hit
TCCGGGATGCGAGTTT38300.1048184178275603No Hit
GTCCTGACTTTACGAC38010.10402475356724716No Hit
TGAGATAGACTCATCC37780.10339529570561952No Hit
TTCTATGACCAACGCC37730.1032584570400483No Hit
AGAACATGAAAGCTAA37460.10251952824596366No Hit
GTTGCTAGACGCTCGA37410.10238268958039243No Hit
CGGTTCACTGGAATGC36910.10101430292468017No Hit
AGGTTACTGGTCCCGA36910.10101430292468017No Hit
GGGTCGTACCGCTCGA36820.10076799332665197No Hit
CCTTCTTTGGATCATC36760.1006037869279665No Hit
GTAAGAAACGACAAAT36710.10046694826239527No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)