FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004950187

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004950187
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2872537
Sequences flagged as poor quality0
Sequence length8
%GC62

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGAGCA280260497.56546216811132No Hit
GTGGCGCA173870.6052837613579912No Hit
GGGGAGCA69270.2411457189237249No Hit
GTGGAGCC47540.1654983034161092No Hit
GTGGAGCT42700.14864908615624448No Hit
GAGGAGCA39690.13817054401736165No Hit
GTGGAGAA39320.13688248402022324No Hit
GTGGACCA32410.11282709326285441ABI Solid3 EF1 alpha Antisense Primer (100% over 8bp)
GTGCAGCA32100.11174790785984653No Hit
GTGGAGGA31270.10885847597437387No Hit
GTGGTGCA31080.10819703975962712No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)