FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004950190

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004950190
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17283666
Sequences flagged as poor quality0
Sequence length34
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGAGAC19500.012.06307622
CGAGACA21050.011.04213923
CTTATAC25100.010.5463191
ATCTCCG22600.010.46757410
CCCACGA22750.010.21500119
CACGAGA23400.010.11213621
TCTCCGA25900.09.29592711
TATACAC39100.09.1650493
CCACGAG26700.08.96621520
TTATACA35750.08.9276392
TAGACTG23450.08.8944435
TATACTG22700.08.324985
TCCGAGC32250.08.11663213
TACACCG7650.08.051315
CTTAGAC16200.08.0359923
GTGTAGA23950.07.9532791
CTAGTAC10500.07.86564353
TATAACG2851.201779E-67.85874032
TAGACAG33000.07.8475325
TACACAT46350.07.7919345
Produced by FastQC (version 0.11.5)