FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004950194

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004950194
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17283666
Sequences flagged as poor quality0
Sequence length34
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG227030.13135523447398254No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG20500.08.8072965
TAGACCG5350.08.6331025
TAGACAG29100.08.6092795
TATACCG3951.2732926E-118.5039545
GTGTATA27850.08.5010441
CTAGTAC10900.08.4743283
TCTATAC15750.08.1751383
TAGACTG22600.08.1747075
TCTACAC21550.08.1180293
CGTAGAC6850.07.96823023
CTAGGAC25700.07.8962843
TCTAGAC20650.07.8618693
GTCTAAC12000.07.7050151
GTTCTAG23000.07.61365181
TGGACTG30100.07.5792785
ATACCGT4451.8007995E-107.5488476
TAGGACC18000.07.4641494
CTTAGAC18250.07.36200673
TGCACCG17350.07.34088855
TACACAG40150.07.32049565
Produced by FastQC (version 0.11.5)