FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004950204

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004950204
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3804254
Sequences flagged as poor quality0
Sequence length8
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCACTCCT370393097.36284696027133No Hit
TCACTCCA223810.5883150809593681No Hit
ACACTCCT166600.4379308006247743No Hit
GCACTCCT139520.36674733075131155No Hit
TCACACCT62870.16526236155629986No Hit
TCACTCAT53030.13939658077510073RNA PCR Primer, Index 45 (100% over 8bp)
TCACTACT50810.13356100828178138No Hit
TCCCTCCT45810.12041782699052167No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)