FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004950205

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004950205
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3740498
Sequences flagged as poor quality0
Sequence length8
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTGATC364696397.49939713909752Illumina PCR Primer Index 9 (100% over 8bp)
ATCTGATA209330.5596313645936984No Hit
ATCTGCTC127330.34040921823778547No Hit
ATCAGATC99240.26531226590683915Illumina PCR Primer Index 9 (100% over 8bp)
ATATGATC75350.20144376497461033No Hit
ATCTGAAC66620.17810462671013325No Hit
AACTGATC61390.16412253127792076No Hit
ATCTCATC49730.1329502114424336No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)