FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004950210

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004950210
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1026931
Sequences flagged as poor quality0
Sequence length16
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATGAATGACTCCTAC57210.5570968253952797No Hit
CTCCTAGTGAACGTAA53410.5200933655717862No Hit
CGACCAGACGCGCTGT48970.47685774409380965No Hit
TAGCTTTACCCTATCC46770.45543468840652396No Hit
CCAGGACTGTAACTCG35840.3490010526510544No Hit
ATCTGTACTGGGACTA33890.33001243511005124No Hit
GTTCTCGACCGTAGAG31940.31102381756904796No Hit
CTTCCTTTGGCTCAGC31710.3087841344744681No Hit
CGATCAAACCCGGCTA30220.2942748831226246No Hit
AGACCATTGAATCCAC27250.2653537579447889No Hit
GGGGGGGGGGGGGGGG26770.2606796367039266No Hit
TGCTGCTACTTTCTTG25640.2496759762827298No Hit
ACAGCCGACTTGCGTA24350.23711427544791225No Hit
AACTTACCTCACCTAA22840.22241026904436617No Hit
CGCGTTGGAGCGGTTC21540.20975119068369735No Hit
GTAACTGTGTGAAAGT19770.1925153686080175No Hit
AGTGGAGTGAGTTGAA19740.19222323603046357No Hit
ATCGAAAGATTTCCGC19580.1906651956168428No Hit
CCCTGAACTAGCTTTG19240.18735435973789863No Hit
GGCAACAGAATAGCAA18790.18297237107459022No Hit
CAATTCGGATAACGGG18540.18053793292830772No Hit
GGGTAGAACCAGTCTA17060.16612605910231554No Hit
AAGAACCCTTAACCCA16950.16505490631795125No Hit
CGCGATCGATATCTGG16420.15989389744783242No Hit
TTCCTAAACTAATGCC16240.15814110198250905No Hit
TCCGGGAACGTTCTCC16060.15638830651718566No Hit
TTTAGTCTGAGCAATA15920.15502502115526748No Hit
GTGAAGCGATCCCAGG15850.1543433784743084No Hit
TGCAGATACTGCCACG15660.15249320548313372No Hit
TGGAACACTCCTAGTA15500.15093516506951293No Hit
AGTCCTGCTCGAACGA14970.14577415619939413No Hit
CTTTAACTGTCCGTAA14670.14285283042385516No Hit
TCTCAGAGATGCATGT14390.1401262597000188No Hit
TGATAGAGAGACTGTT14350.1397367495966136No Hit
CACATCCTGAGCCTAT14170.13798395413129022No Hit
CATAGGTACAAGGTGC13990.13623115866596686No Hit
TGCAGTATGACACCGC13970.13603640361426425No Hit
CTATTGGGACTTGTGA13890.13525738340745386No Hit
AGGTGTGGATCATGTG13880.13516000588160254No Hit
TCAAAGCACTCTGCAA13860.13496525082989996No Hit
CTTAGATCTTGTCACA13590.13233605763191492No Hit
TACGTTTACTGCACCG13270.12921997680467334No Hit
GGTCTAAACTCCTACC13110.12766193639105258No Hit
TGAACTGTGAACGTAA13010.12668816113253958No Hit
ATTTCTGACGGGTAGT12950.1261038959774318No Hit
GCAACTTGAAGGTGCA12850.1251301207189188No Hit
TGGCAATTGAGTGGTG12800.1246432330896623No Hit
ATGTACGTGTACTTTC12750.12415634546040581No Hit
TGAGGGATGTACGTTG12720.12386421288285192No Hit
AGAGTACCTTAATCGC12640.12308519267604152No Hit
CGAGTCTCTGCTACGC12310.11987173432294868No Hit
GAGTGTTACACCTGCG12210.11889795906443568No Hit
GGGTGTGCTCGCAAAG12150.11831369390932789No Hit
TACTGTTTGCTTAAGC12130.1181189388576253No Hit
TTCTTAGACGTCCCGA12060.1174372961766662No Hit
CTACAACGAATCTGTG12030.11714516359911231No Hit
TCCATTTGAAATCTGC11980.11665827596985581No Hit
CACCAGGTGCGCCACA11900.11587925576304542No Hit
TCTATGGGATGCTCAA11780.11471072545282984No Hit
CTCAATTTGGAATCTC11780.11471072545282984No Hit
GGGATGTGACTGGAAG11560.11256841988410127No Hit
TCGGACCGATAGGCGA11500.11198415472899348No Hit
GGTCGCATGGTACTAC11470.11169202215143959No Hit
CACATTACTGATGGAC11450.11149726709973698No Hit
GTAATCTCTACACAAA11340.11042611431537269No Hit
TGCTAGTGAAGGCGTA11270.1097444716344136No Hit
CATTCTATGCCTAGCG11160.10867331885004933No Hit
TTGCGGTCTTTTGGGT11130.10838118627249542No Hit
TCCGGGTGAACACCTA11110.10818643122079283No Hit
AGCCAATTGTTATGGC11080.10789429864323893No Hit
CTAGCTCACGAATAGT10920.10633625822961815No Hit
AGACTACTGGCCCTAG10910.10623888070376686No Hit
TAGTACCGAACGAGCA10780.10497297286769997No Hit
ATGTCCAGACCGCTAG10770.10487559534184868No Hit
CGAGTCTCTTGGAACG10670.10390182008333569No Hit
CATAGTCGAAGATTCC10620.10341493245407919No Hit
CTTTCGTCTCGAACGA10590.1031227998765253No Hit
ACCTCAGCTAATCCGT10520.10244115719556621No Hit
CTAACAGTGCATGATC10460.10185689204045842No Hit
TGTAAGCACGCGCTGT10460.10185689204045842No Hit
AGGCGATGACCTGCGA10410.10137000441120192No Hit
TCGTTGCTGTATTGCG10400.10127262688535063No Hit
AGCTTGTGATTGCGTA10400.10127262688535063No Hit
ACCAAGTTGAAGCTAA10390.10117524935949933No Hit
TGCGCAGACATCAGGG10360.10088311678194542No Hit
GGCTGACTGAAACTGA10270.10000671904928374No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)