FastQCFastQC Report
Sun 4 Sep 2022
EGAF00004950217

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004950217
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4352077
Sequences flagged as poor quality0
Sequence length16
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTAGTGAACGTAA229990.5284603190614504No Hit
CGACCAGACGCGCTGT210630.4839758120088408No Hit
TAGCTTTACCCTATCC199600.4586315913068633No Hit
GATGAATGACTCCTAC186750.42910545930138644No Hit
CCAGGACTGTAACTCG148100.3402972879386095No Hit
ATCTGTACTGGGACTA138810.3189511582630546No Hit
CTTCCTTTGGCTCAGC137950.31697509028447796No Hit
GTTCTCGACCGTAGAG133630.30704879532232543No Hit
CGATCAAACCCGGCTA127250.2923891282254427No Hit
AGACCATTGAATCCAC116280.2671827727312729No Hit
TGCTGCTACTTTCTTG111590.25640630898763966No Hit
ACAGCCGACTTGCGTA104650.2404598999512187No Hit
GGGGGGGGGGGGGGGG101960.23427894313450795No Hit
GGCAACAGAATAGCAA100430.23076338033541227No Hit
AACTTACCTCACCTAA94110.21624157844633723No Hit
CGCGTTGGAGCGGTTC88800.20404050755535807No Hit
ATCGAAAGATTTCCGC87510.20107640558749304No Hit
GTAACTGTGTGAAAGT83120.1909892678828982No Hit
AGTGGAGTGAGTTGAA83060.19085140267509054No Hit
CCCTGAACTAGCTTTG81530.18733583987599484No Hit
CAATTCGGATAACGGG80450.1848542661354567No Hit
GGGTAGAACCAGTCTA72820.16732240720924743No Hit
AAGAACCCTTAACCCA71250.16371493427161332No Hit
TTCCTAAACTAATGCC70860.1628188104208634No Hit
TTTAGTCTGAGCAATA70170.16123336053107518No Hit
GTGAAGCGATCCCAGG68360.15707442676221034No Hit
CGCGATCGATATCTGG67250.15452392041776833No Hit
TGGAACACTCCTAGTA66590.15300740313188393No Hit
TGCAGATACTGCCACG64100.14728599700786543No Hit
TGATAGAGAGACTGTT62980.14471251312878886No Hit
CTTTAACTGTCCGTAA62700.1440691421590197No Hit
AGTCCTGCTCGAACGA62630.14390829941657743No Hit
CACATCCTGAGCCTAT61700.14177138869555847No Hit
CATAGGTACAAGGTGC59240.1361189151754438No Hit
GCAACTTGAAGGTGCA59160.13593509489836691No Hit
AGGTGTGGATCATGTG58810.1351308811861555No Hit
TCAAAGCACTCTGCAA58550.13453346528565557No Hit
TCTCAGAGATGCATGT57560.13225868935682894No Hit
TGCAGTATGACACCGC57530.1321897567529251No Hit
CTTAGATCTTGTCACA57500.13212082414902127No Hit
TGGCAATTGAGTGGTG56470.12975413808165617No Hit
TCCGGGAACGTTCTCC55790.12819166572650253No Hit
CTATTGGGACTTGTGA55560.12766318242990646No Hit
ATGTACGTGTACTTTC55260.12697385639086808No Hit
AGAGTACCTTAATCGC54740.12577902458986825No Hit
TACGTTTACTGCACCG54610.12548031663961828No Hit
CGAGTCTCTGCTACGC54560.12536542896644523No Hit
TCTATGGGATGCTCAA53940.12394082181909924No Hit
ATTTCTGACGGGTAGT53830.12368806893811851No Hit
TGAACTGTGAACGTAA53640.12325149578006087No Hit
TTCTTAGACGTCCCGA52090.11968997791169594No Hit
TACTGTTTGCTTAAGC51990.11946020256534984No Hit
GGTCTAAACTCCTACC51680.11874789899167684No Hit
GGGTGTGCTCGCAAAG50550.11615143757796566No Hit
TCCATTTGAAATCTGC49800.11442812248036972No Hit
CTACAACGAATCTGTG49370.11344008849108139No Hit
GCATGATACTTGCGTA49160.11295756026375452No Hit
CATTCTATGCCTAGCG48760.11203845887837002No Hit
GAGTGTTACACCTGCG48480.11139508790860089No Hit
TTGCGGTCTTTTGGGT47960.11020025610760105No Hit
CACCAGGTGCGCCACA47900.11006239089979336No Hit
GGTCGCATGGTACTAC47800.10983261555344724No Hit
CTCAATTTGGAATCTC47710.10962581774173571No Hit
CTAGCTCACGAATAGT47530.1092122221183127No Hit
AGCCAATTGTTATGGC47400.10891351416806275No Hit
CTTTCGTCTCGAACGA46760.10744295195144755No Hit
AGACTACTGGCCCTAG46420.10666171577387072No Hit
GTAATCTCTACACAAA46270.10631705275435153No Hit
GGGATGTGACTGGAAG46200.10615621001190925No Hit
TGCTAGTGAAGGCGTA45850.10535199629969783No Hit
CGAGTCTCTTGGAACG45740.1050992434187171No Hit
TGTAAGCACGCGCTGT45610.10480053546846713No Hit
ACCAAGTTGAAGCTAA45270.1040192992908903No Hit
TGCGCAGACATCAGGG45110.1036516587367365No Hit
TCGGACCGATAGGCGA44990.10337592832112116No Hit
CACATTACTGATGGAC44960.10330699571721733No Hit
ATGTCCAGACCGCTAG44800.10293935516306352No Hit
CTAACAGTGCATGATC44620.1025257595396405No Hit
ACCTCAGCTAATCCGT44030.10117008499619838No Hit
CATAGTCGAAGATTCC43710.10043480388789076No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)