FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004950222

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004950222
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16203524
Sequences flagged as poor quality0
Sequence length34
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG17200.08.8727425
GGACCGT10150.08.2764666
TACACCG8350.08.2161695
TACACTG27750.08.1735715
TCTATAC13350.07.9705713
GTATAGA14000.07.90094331
GTACTAG10300.07.8844361
GTACTAA11400.07.86058661
TAGACTG21400.07.8510415
ACACCGT11600.07.84540136
GTATTAG16500.07.80699251
GTATAGG14400.07.7787061
CTAGCAC24750.07.75001343
TACACAG38550.07.7359745
CTAGTAC9600.07.7296843
CGTCAGA26300.07.71955251
TAGGACC16950.07.6819064
CTTACAC22400.07.625513
GTCTAAG16850.07.56173321
TGTACTG24650.07.4975055
Produced by FastQC (version 0.11.5)