FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004950228

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004950228
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2981995
Sequences flagged as poor quality0
Sequence length16
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACAGCCGACCAGGGAT521751.749667588309169No Hit
GAAGCAGTGGAACCTT170790.5727373788353098No Hit
GTTCTTCCTAACGTAA160210.5372577754154517No Hit
CATGGAGGATCGACAT123160.4130120942523378No Hit
CGCTTAAGATAATAGG123010.41250907530026043No Hit
TGTCCCATGAGACAAT120000.40241516166190755No Hit
AAAGCATGATCTTTGT113090.3792427552695427No Hit
TGAGCGCACAGGACAC105130.3525492162126362No Hit
TGTCGAAACTTCGCAA100640.3374921822471198No Hit
TAATTGCCTGTCTGGC99260.33286440788800786No Hit
GATACGTACCGCTCGA92540.310329158834941No Hit
GGAAGGACTAGGTAAT91980.3084512214138521No Hit
GGGGGGGGGGGGGGGG88900.29812256559786315No Hit
CATGTCAGAGGTTTGT79890.26790789387641495No Hit
AATGATGGAAAGCTAA78320.262642962178005No Hit
TCGACCTTGTTTCGCC74390.24946386563357753No Hit
GTGAGATCTCGATTAC68070.22827000045271706No Hit
GCTATGATGACCTGCG63250.21210632479263045No Hit
ATGGGTACTTGGCTGC61670.20680785849741534No Hit
AAACGACCTAGGGAGG59220.19859188228015137No Hit
CAGCTCACTGTTTACG56990.19111366719260092No Hit
AGCCTACCTTAACTCG56460.1893363335619275No Hit
TGTTCCTCTTTATGGC56400.18913512598109652No Hit
TTGCGATGACATTCAG56280.18873271081943463No Hit
CTGCCTTGACCATAAC52030.1744805071772421No Hit
TAACCAGACATCACAC50530.16945031765646823No Hit
AGCTTGTGACTCACTC50500.16934971386605277No Hit
CAATGTGACCAACGCC50490.1693161792692476No Hit
CACAGCTCTGGACTGA49370.16556030442706982No Hit
CCTGTTGACCCTAGTA48870.16388357458681185No Hit
CATGCAAACGAAGGCT48060.16116727224559396No Hit
CATGATGTGTCATGTG47350.1587863158724277No Hit
ATGGGTACTTTCGGGC46880.15721018982258522No Hit
CTATTCAACCGAACGA46220.15499690643344471No Hit
ATGCCGCGATGCCACG45870.15382319554526416No Hit
CATTAGTCTATTCTGG44960.1507715472359947No Hit
TTGTTTGTGATACGCT44380.14882654062129547No Hit
CACTATACTACGCCAC43300.1452048041663383No Hit
ATTAGCGTGGTCGGTC43040.14433290464940418No Hit
AGAACGCGAGGAATAT42470.1424214326315101No Hit
GCAGATACTCGGCTCA41130.13792779665961882No Hit
GCATCTCTGAGCCTAT41060.1376930544819827No Hit
ATAGGAAACAGACTTC40930.13725710472351563No Hit
CCGCTATTGTTCGCAA40630.13625106681936086No Hit
AACCAACACCGGAATG39960.1340042488334152No Hit
TGGGCCATGCGTTCTC39880.13373597205897395No Hit
GGGTCGTACCGCTCGA39860.13366890286536362No Hit
AGCTCGGACCTTAAGC39850.13363536826855846No Hit
GGGATCAGATGAGTAA39810.13350122988133784No Hit
CCCAATGCTGGAATGC39780.13340062609092235No Hit
TTGACGTGACCTCAAA39400.1321263114123263No Hit
AACGTCCCTTACAGAA39270.13169036165385925No Hit
CTGCCATTGCACAGTT38490.12907466310305685No Hit
TTGTAACCTCATCCTC38380.1287057825382001No Hit
CTGATTACTGCTTTGA38100.12776681382765565No Hit
TGGAGTTACAAGTCTC38040.1275656062468247No Hit
TGTTTGTTGCGCTCGA37610.12612361858420287No Hit
ATAGCCAACATGGCCC37520.1258218072129564No Hit
GATACCATGTCTGGAT37190.12471516551838617No Hit
CGCTTACTGGCCACGT36990.124044473582283No Hit
CGATCAAACCGATCGC36650.12290429729090761No Hit
CCTACGGGATGGAACG36270.12162998261231156No Hit
ATCTTACTGCGCACTG36180.12132817124106511No Hit
CGCTGAGTGTTAGCTG36010.12075808309537743No Hit
CGCTTCCCTGCGCTGT35440.11884661107748336No Hit
TCCAAAGTGAAGGCCA35060.11757229639888732No Hit
TGGGTGCACTTCTGAG34940.11716988123722542No Hit
TTCTAACACTACGTTG34710.11639858551070675No Hit
TCAGGCTCTTAAAGCA34580.11596263575223968No Hit
GTAAGAAACGACAAAT34490.11566082438099327No Hit
GCGTCGATGCTTGAAC34390.11532547841294168No Hit
CTCGGATCTTTCTGAG34050.11418530212156626No Hit
TACAACCGATTCCATT33820.1134140063950476No Hit
GCATATCGAGCCGCTA33630.11277684905574958No Hit
CGAGTCTCTTACTTTC33370.11190494953881545No Hit
GCGACAAGAAAGGCTG33140.1111336538122968No Hit
AGTGGTACTCAACGCC33110.11103305002188132No Hit
AGAACAATGAGTCGGA33070.11089891163466069No Hit
CCTCCCTTGTTTCGCC32780.10992640832731108No Hit
CCTTCAGTGTAGCTTG32720.10972520074648012No Hit
CATGTTACTAACGTGG32640.10945692397203886No Hit
TACGCCTTGGGTTGAG32520.10905450881037695No Hit
CTTAGGCCTTATGACC32270.10821614389024797No Hit
AACAGCTACTCGAGAG32180.10791433251900154No Hit
GGTCAGAGAACCAGTT32060.10751191735733964No Hit
AACCACTGAAAACATG32030.10741131356692415No Hit
ATCGCAATGTAACTCG31970.1072101059860932No Hit
ATTGAGAGATGATCAC31900.1069753638084571No Hit
GGTAGCCTGTGCCACG31870.10687476001804161No Hit
GCATAGTACGCTCCTT31660.10617053348513326No Hit
GTGTACTGAATCACAC31440.10543277235541977No Hit
GGGCTGACTAATACAG31430.10539923775861462No Hit
TATCTCCCTGGAATTA31110.10432613066084953No Hit
TGAGATAGACTCATCC30830.10338716195030509No Hit
TGGTCCTTGTTTCTGT30790.10325302356308445No Hit
TTCTATGACCAACGCC30750.10311888517586382No Hit
AGCCGGTACGCGCAAA30750.10311888517586382No Hit
GTTGCTAGACGCTCGA30570.10251526243337095No Hit
ACCTCCATGGAATTGA30550.10244819323976062No Hit
GAAAGTAACCCTGCGA30360.10181103590046262No Hit
CATGTCAGAACAGGTG30340.1017439667068523No Hit
TTCACGCTGTGGGCAA30240.10140862073880069No Hit
AAAGGATACTGCTGGC29980.10053672122186656No Hit
AGAACATGAAAGCTAA29880.10020137525381498No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)