FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004950246

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004950246
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2456518
Sequences flagged as poor quality0
Sequence length16
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATGAATGACTCCTAC149030.6066717198896976No Hit
CTCCTAGTGAACGTAA126860.5164220249963566No Hit
TAGCTTTACCCTATCC113490.46199539347971397No Hit
CCAGGACTGTAACTCG85990.350048320427532No Hit
ATCTGTACTGGGACTA83930.3416624669552594No Hit
CTTCCTTTGGCTCAGC77090.3138181767851894No Hit
GTTCTCGACCGTAGAG75810.30860754938494245No Hit
CGATCAAACCCGGCTA70930.28874203242150065No Hit
TGCTGCTACTTTCTTG62150.25300038509793127No Hit
GGGGGGGGGGGGGGGG59780.2433525828021614No Hit
ACAGCCGACTTGCGTA58760.2392003640925896No Hit
AACTTACCTCACCTAA53000.21575254079147801No Hit
CGACCAGACGCGCTGT52280.21282156287883908No Hit
CGCGTTGGAGCGGTTC51260.20866934416926722No Hit
ATCGAAAGATTTCCGC49950.2033365926893269No Hit
GGCAACAGAATAGCAA48050.19560206764208526No Hit
GTAACTGTGTGAAAGT47370.19283392183570405No Hit
CCCTGAACTAGCTTTG46470.18917019944490535No Hit
AGTGGAGTGAGTTGAA46440.18904807536521206No Hit
CAATTCGGATAACGGG45190.18395957204465832No Hit
GGGTAGAACCAGTCTA40070.16311706244367025No Hit
GTGAAGCGATCCCAGG39860.16226219388581725No Hit
TTCCTAAACTAATGCC39460.16063387282324004No Hit
CGCGATCGATATCTGG39230.15969758821225816No Hit
TTTAGTCTGAGCAATA38490.15668519424649036No Hit
AAGAACCCTTAACCCA38460.15656307016679707No Hit
TCCGGGAACGTTCTCC37990.15464979291826886No Hit
TGGAACACTCCTAGTA37260.15167810697906547No Hit
TGCAGATACTGCCACG36780.14972412170397284No Hit
TGATAGAGAGACTGTT35230.14341437758648623No Hit
CTTTAACTGTCCGTAA35070.14276304916145535No Hit
TGCAGTATGACACCGC34820.1417453484973446No Hit
AGTCCTGCTCGAACGA34700.14125685217857145No Hit
AGGTGTGGATCATGTG34630.14097189599262044No Hit
CACATCCTGAGCCTAT34490.14040198362071843No Hit
CATAGGTACAAGGTGC34210.1392621588769144No Hit
TCTCAGAGATGCATGT33240.1353134803001647No Hit
CTATTGGGACTTGTGA32980.13425507160948952No Hit
GCAACTTGAAGGTGCA32880.13384799134384523No Hit
ATTTCTGACGGGTAGT32580.13262675054691234No Hit
CTTAGATCTTGTCACA32520.13238250238752575No Hit
TGGCAATTGAGTGGTG32170.1309577214577707No Hit
TACGTTTACTGCACCG31900.1298586047405311No Hit
TGAACTGTGAACGTAA31130.12672408669507002No Hit
TCAAAGCACTCTGCAA31030.1263170064294257No Hit
AGAGTACCTTAATCGC30940.12595063419034586No Hit
ATGTACGTGTACTTTC30940.12595063419034586No Hit
TGAGGGATGTACGTTG30900.12578780208408813No Hit
CGAGTCTCTGCTACGC30820.1254621378715727No Hit
GGTCTAAACTCCTACC30640.12472939339341295No Hit
TCTATGGGATGCTCAA30170.12281611614488475No Hit
TTCTTAGACGTCCCGA29790.12126921113543641No Hit
GGTCGCATGGTACTAC29620.12057717468384112No Hit
TCCATTTGAAATCTGC29330.11939664191347266No Hit
TACTGTTTGCTTAAGC28470.11589575162893168No Hit
CTCAATTTGGAATCTC28240.11495946701794979No Hit
GGGTGTGCTCGCAAAG28200.11479663491169208No Hit
CATTCTATGCCTAGCG28190.11475592688512766No Hit
CTACAACGAATCTGTG28160.11463380280543435No Hit
GCATGATACTTGCGTA28090.11434884661948334No Hit
GAGTGTTACACCTGCG27940.11373822622101691No Hit
GGGATGTGACTGGAAG27600.11235415331782629No Hit
CTAGCTCACGAATAGT27580.11227273726469743No Hit
TTGCGGTCTTTTGGGT27140.1104815840958625No Hit
TCCGGGTGAACACCTA26960.10974883961770278No Hit
CACCAGGTGCGCCACA26860.10934175935205849No Hit
CACATTACTGATGGAC26700.10869043092702761No Hit
AGACTACTGGCCCTAG26690.10864972290046318No Hit
CGAGTCTCTTGGAACG26480.10779485434261014No Hit
AGCCAATTGTTATGGC26440.10763202223635243No Hit
TCGGACCGATAGGCGA26340.10722494197070813No Hit
CTTTCGTCTCGAACGA26340.10722494197070813No Hit
GTAATCTCTACACAAA26080.10616653328003296No Hit
TGCTAGTGAAGGCGTA25970.10571874498782423No Hit
CATAGTCGAAGATTCC25930.10555591288156652No Hit
GGCTGACTGAAACTGA25290.102950599181443No Hit
TAGTACCGAACGAGCA25190.10254351891579871No Hit
TGTAAGCACGCGCTGT25120.1022585627298477No Hit
ACGAGGATGAACTAGA25010.10181077443763896No Hit
ATGTCCAGACCGCTAG24970.10164794233138125No Hit
CTAACAGTGCATGATC24870.10124086206573694No Hit
ACCAAGTTGAAGCTAA24700.10054882561414163No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)