FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004950257

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004950257
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16700583
Sequences flagged as poor quality0
Sequence length34
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG180040.1078046197548912No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGTATA35850.010.1623231
GTATAAG29550.09.049113
TATACTG19550.08.5926185
TATACCG3455.7480065E-108.5210125
TACACCG6100.08.4910795
TATAAGA36000.08.4383214
TAGACAG28300.08.3597125
TACACTG28700.08.1456475
GTCCTAC13250.08.1429851
ATAAGAG39450.08.0550795
TGTATAA35550.07.9563412
GCACCGT14500.07.62686736
GTGTAGG23950.07.60583071
CTAGACT22550.07.51168974
CCTACAC24450.07.5010573
TAGACTG22650.07.3547815
CTACACT27050.07.2971064
TCTACAC19450.07.2699563
TAGGACC15300.07.22827154
CTAGGAC22400.07.1875333
Produced by FastQC (version 0.11.5)