FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004950268

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004950268
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17311110
Sequences flagged as poor quality0
Sequence length34
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGAGAC18950.011.89628622
CACGAGA21400.010.5337821
CTTATAC24300.010.4896621
TATACAC38000.09.9091853
CCCACGA22750.09.90720919
TCTCCGA25150.09.29477611
TAGACAG30700.09.0281465
ATCTCCG25200.08.998651510
TATACTG21550.08.5742955
TAGACCG6400.08.5301455
GACTCTA26400.08.434525526
CTAGACA28800.08.4086324
CCACGAG28450.08.16869620
TGTACCG6200.08.1279795
CCTACAC27900.08.1279333
TACACAT42600.08.1163195
CTAGGAC27600.08.0134083
TTATACA33950.07.9990092
CTATACT20800.07.9412794
GTCTAGA21000.07.9364091
Produced by FastQC (version 0.11.5)