FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004950272

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004950272
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17311110
Sequences flagged as poor quality0
Sequence length34
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG229430.13253338462987063No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGTATA33650.08.8668131
TACACTG29150.08.3064855
TCTATAC13950.08.12634853
TATACTG20600.08.0851765
TAGGACC18150.08.019464
TAGACAG27650.07.94720275
GTGTAGG28750.07.8931461
CTACCCT30350.07.8393244
TAGGACT23950.07.7720314
GTCCTAG26750.07.75016361
TAGACTG22600.07.7412545
TCTACGG5850.07.6572082
GTATAGA15650.07.60812331
GTAGGAC17150.07.589333
CTAAGAC21500.07.55099443
TTAGGAC17850.07.5269263
TCTAGAC18450.07.5097153
CTAGGAC26150.07.49274543
TGCACCG17750.07.49091675
CTATACT19300.07.46910054
Produced by FastQC (version 0.11.5)