FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004950273

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004950273
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4563141
Sequences flagged as poor quality0
Sequence length8
%GC62

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCAGTCC438885496.18054756581049No Hit
CCCAGTCC530391.1623353299843244No Hit
ACCAGTCA310880.6812851060267477No Hit
ACAAGTCC115370.25283023250870396No Hit
ACCAGACC105410.2310031620762979No Hit
ACCACTCC100970.22127302224498432No Hit
ACCCGTCC79090.17332359442761028TruSeq Adapter, Index 16 (100% over 8bp)
ACCAGTCG62100.13609046926229104No Hit
ACCAGTAC61890.13563025994594513No Hit
AACAGTCC48970.10731642962599666No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)