FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004950287

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004950287
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15594974
Sequences flagged as poor quality0
Sequence length34
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG186350.11949362660046757No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACCG5300.08.9811565
CTACACT24650.08.6322474
TACACCG7750.08.4903465
TCTACAC19950.08.3501233
TACACTG25850.08.0696545
TATACAG21050.07.98101955
GTGTTAG23850.07.9286441
GTCTAGA18200.07.9271921
GTGTAAG20150.07.924711
AGACCGT9650.07.8342446
GTACTAG9650.07.6931131
GTATAGG12850.07.63042071
CTAAGAC19300.07.6158933
TAGACTG19900.07.59801055
CCTACAC23250.07.58641153
TGGACTG23500.07.56597765
TTAGGAC16500.07.4659933
TATACTG17550.07.41882375
GTCTTAG17750.07.41793541
GTCCTAA16250.07.41307971
Produced by FastQC (version 0.11.5)