FastQCFastQC Report
Sun 4 Sep 2022
EGAF00004950289

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004950289
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3971170
Sequences flagged as poor quality0
Sequence length16
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCACTGTCTCTTTGTT406581.0238292493144336No Hit
TGCTGCTACCGTTCTC375610.9458421573490935No Hit
AAACGACCTCTTCAAG374240.9423922924478176No Hit
ATGGGTTACAATCGAA358080.9016989955101393No Hit
TATGCTTTGGACTGTT351210.8843993080125002No Hit
GCCTCTTGATCTGTGA346550.872664731049036No Hit
AAAGCGGACTCGGGCT324490.8171143516898042No Hit
TCCAAAGTGCTCACGG314320.7915047706343471No Hit
TAGTGCATGAACCCGC306660.7722157449819574No Hit
GCAGGTCTGGCTGTTA294790.7423253096694425No Hit
TAACTCACTGGTGTCC293370.7387495372900178No Hit
TGTGGCTCTTACGATC285870.7198634155677042No Hit
GAAGTATGAACAGGTG281830.7096900913332846No Hit
TTCCCATCTCTTTGTT248930.6268429707114024No Hit
GGAACTACTACCAAGG245200.6174502728415051No Hit
GAGCCTGACCGAGTTT243690.6136478670014126No Hit
TCACCTGACTCAATGC241540.6082338454410161No Hit
ATGTCCAGAGTCGGTC235210.5922939587073834No Hit
TGAGAATGAGCAGTTT230960.5815918230647391No Hit
CTAGAGACTAATCTGC230090.5794010329449507No Hit
GAAGTAATGAGTCTGT225220.5671376445732618No Hit
TCGTATACTGCTAATC221960.5589284769979629No Hit
TTCGTCCGACCTGTGG217950.5488306972504324No Hit
TGCGGTGACCTTAGTG214340.5397401773280922No Hit
TCACACAGATTGGGAG214040.5389847324591998No Hit
ATTCCTAACTGCTACA209560.527703422417071No Hit
TCCGCGAGAGAACAGC206190.5192172583898448No Hit
ACTGATTCTCGGCTCA206020.5187891729641391No Hit
TGGCCTGGACCCGAGT203150.5115620837184004No Hit
AGGTCGAGAAGGGAGG201190.5066265105749691No Hit
TGGACCTCTAGGGAGG195820.49310404742179254No Hit
CCATGATGATTCGGGC187660.47255594698791537No Hit
GCAATCATGGAACGTG179040.45084949775506966No Hit
ACGAGGATGGAGCTGA175740.44253960419725175No Hit
TTCGATGGAAATCCTG172790.435111062986475No Hit
TCCCAGACTGTCTTGA169250.426196813533543No Hit
AAGGTGCGAACAGATA166210.4185416388620986No Hit
TTTCGTAACAGTACGG161800.4074365992893782No Hit
TGGTCCTTGGAGAGGG159710.4021736667027601No Hit
TCTCTCTACTCTGGAT156220.3933853247279769No Hit
GCGGTTCGAGTACGTT155340.3911693531125588No Hit
TCCTTCACTTGTCACA145760.3670454802992569No Hit
TCGCCGAGAAGGTAAT138280.34820972156820285No Hit
AGCCCAGTGAAAGTAC137850.34712691725612355No Hit
TTCGATGGACAGGTGA133870.33710468199548244No Hit
TGATATCCTCGATGTT133270.33559379225769737No Hit
AGCTGCTCTTGTCACA132670.3340829025199123No Hit
AACCCTCACGCGGTTC129060.324992382597572No Hit
AACTCCCTGCTTCACC128660.32398512277238195No Hit
CTTTCGGTGCTTTGTT127010.31983017599347296No Hit
CCCTTGCCTCCTACGG124340.3131067166603293No Hit
ATGGCCGTGATACGCT123040.3098331222284616No Hit
TCGGCTTACCTCCTAC117790.29661283702284214No Hit
CTAACTGGAACCTTGA114780.2890332068382869No Hit
TGTTTGTTGCAAGTTC114280.28777413205679936No Hit
AAACACGCTGCAGTTT113010.28457608211182095No Hit
ACCCTTGTGGGCCATC110720.2788095196126079No Hit
AAATAGGACTCTATCT109810.2765180035103005No Hit
TGGCCTTTGCGAAAGC109610.2760143735977055No Hit
TACAACGTGAGCAGGT109080.2746797543293286No Hit
TCAAAGGCTCTTAGTG106270.26760375405736847No Hit
CAATGTGTGGTTGACT104600.2633984442872No Hit
GGGGGGGGGGGGGGGG104040.2619882805319339No Hit
CTTTCGGTGAGCCATT100370.25274667163581516No Hit
TGAACTGTGTGCTGGC95670.24091136868983196No Hit
CAGCCGATGTAATAGG93910.23647942545899572No Hit
AGTGTTCGATTCGCCT93550.23557289161632464No Hit
GAGGTTGGAGGAATAT91890.23139276334178593No Hit
GATAAGAACATTGGTC84670.21321172349710538No Hit
ACAGCCGACAAACATG83860.2111720223510955No Hit
GGCTTGCGAATACGCT83680.21071875542975999No Hit
GTGCTAATGGGAGTCA82470.20767179445856007No Hit
CGACGTTCTTGCTACA81620.2055313673300312No Hit
TTGGGAGGAATCTGTG81070.20414638507039484No Hit
GTAAAGCCTGAAATCA79820.20099869811667595No Hit
TGTACTGGAAATGCTC79800.20094833512541646No Hit
TCTTGAACTCGTACAT74230.1869222420596449No Hit
AGGGACCCTAATCCTG73030.18390046258407472No Hit
TAACGAGCTTACTTTC72540.1826665692982169No Hit
GGAACATCTTTACGAC72480.18251548032443837No Hit
GGGTGAAACCGACTAT71060.17893970794501368No Hit
GGACCACTGGCTAGAG70200.17677409932085505No Hit
CGGATGAGAACAGGCA69430.17483512415736419No Hit
TACCACCTGAAACATG68110.17151116673423702No Hit
TACGTGTGAAACGCAG67730.17055426990030648No Hit
AGCTTGTGAACCGTGC65910.1659712376956917No Hit
AACTAGCACTGCCACG65810.1657194227393942No Hit
CATGATGTGCCTAGTA65100.16393153654968182No Hit
ACGTGTTGATCTTTGT64580.16262209877693476No Hit
GGACATTTGGCCGCTA64430.16224437634248848No Hit
TCGGACCGAATGTCCA63550.16002840472707036No Hit
ACTTATCTGTCAGGTT62890.15836642601550677No Hit
TGGTAGGGATGTGCTA60830.15317903791577797No Hit
CTCGGATCTAGTACGG59840.15068606984843258No Hit
AACTACGACATTGCCA59610.1501068954489483No Hit
GTCCGAGTGGTCTTGA58990.1485456427199037No Hit
GTAGATAGAGCTGAGA58890.1482938277636062No Hit
CGTCGTACTTGCGCCA58440.14716066046026738No Hit
TACCACCTGTACTTTC57050.14366043256773192No Hit
AACGAACCTGACACCC57020.1435848880808427No Hit
GGTAGTTTGTCCAGCG56510.14230063180372535No Hit
TTTAGGGACTGGGCAA55920.14081492356157002No Hit
TGAGGAGTGGTCCCAG55370.1394299413019337No Hit
CCTTCAGTGCAACGCC55160.13890112989370892No Hit
AGTTTGCGAGCAGGAT54270.13665997678266104No Hit
GCCATAATGACCGTTA53750.13535053900991395No Hit
CTTTAACTGTTCGCCT53420.13451954965413215No Hit
GTCCGTTACGGATGGT51500.12968470249321987No Hit
CAGGCCATGTCGTAGT50530.127242097417134No Hit
CGATCGGTGCGAGGAT49580.12484985533230762No Hit
GAGAAGAGATGCTACA48840.12298642465570599No Hit
GGGATGTGATGCATGT48220.12142517192666141No Hit
AGAACGCGACACAGTT48180.12132444594414239No Hit
CTTACATTGGAAGGCT48140.1212237199616234No Hit
TGCAATGACTCCGTAA48080.12107263098784489No Hit
CCGTTACTGTTGGTTG46910.11812639599916397No Hit
TCCTCATCTTTCTGGA46810.11787458104286647No Hit
CAAAGACCTGCTTTGA46200.11633850980945162No Hit
GAACCATACTTTGGGT45960.11573415391433758No Hit
AACTACGACGCTGTTA45710.1151046165235938No Hit
GAAGGACACCCAGCTG45190.11379517875084671No Hit
GAAAGTCCTTATCTGG45110.11359372678580872No Hit
GCTGGACACATTGGTC43880.1104964028233493No Hit
AATTCTGCTTAGCGAG43700.11004313590201377No Hit
GGCTTAAACTGCATCA43300.1090358760768237No Hit
TGTCTTCCTGAAATCA43190.10875887962489643No Hit
ATGGCCGTGGGCCAAA43030.10835597569482042No Hit
GCGTTTAGAAGTCTAC42640.10737389736526011No Hit
CCCACCTCTTGACCTA42450.10689544894829484No Hit
CCTGAGGACTGACTCA42150.1061400040794023No Hit
TCGTAGAACCGCACTG42110.10603927809688328No Hit
TCCGTAAGATTACGCA41820.1053090147236205No Hit
ACAAGACCTAAGCTAA40930.10306786161257261No Hit
AGGTGATCTGTTAGTC40890.10296713563005362No Hit
ATAGCCAACATCTGTG40740.10258941319560733No Hit
GCGTTAATGGTACGTT40620.10228723524805033No Hit
TATGGGTACCCTTTGC40250.10135551990974952No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)