FastQCFastQC Report
Mon 12 Sep 2022
EGAF00004950290

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004950290
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17227410
Sequences flagged as poor quality0
Sequence length16
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCACTGTCTCTTTGTT1825901.059880736570384No Hit
TGCTGCTACCGTTCTC1782061.0344329182390155No Hit
AAACGACCTCTTCAAG1724721.0011487507408252No Hit
ATGGGTTACAATCGAA1703940.9890865777270059No Hit
TATGCTTTGGACTGTT1676120.9729378937402663No Hit
AAAGCGGACTCGGGCT1556370.9034265742790124No Hit
GCAGGTCTGGCTGTTA1534860.890940657939876No Hit
TCCAAAGTGCTCACGG1472690.8548528188508894No Hit
TAGTGCATGAACCCGC1385330.8041429326869217No Hit
GAAGTATGAACAGGTG1363730.7916047740200065No Hit
TGTGGCTCTTACGATC1303460.7566198285174615No Hit
TAACTCACTGGTGTCC1289670.74861514296113No Hit
TTCCCATCTCTTTGTT1176120.6827027394135275No Hit
TCACCTGACTCAATGC1123930.6524079940049027No Hit
TGAGAATGAGCAGTTT1096210.6363173570490283No Hit
GGAACTACTACCAAGG1069190.6206330493092114No Hit
GCCTCTTGATCTGTGA1068580.6202789624209327No Hit
GAAGTAATGAGTCTGT1028440.5969788842315822No Hit
ATGTCCAGAGTCGGTC1028150.5968105478420727No Hit
CTAGAGACTAATCTGC1023600.5941694079376992No Hit
TTCGTCCGACCTGTGG1012720.5878538909795494No Hit
TCGTATACTGCTAATC1009020.5857061508375316No Hit
TCACACAGATTGGGAG989440.5743405421940965No Hit
TGCGGTGACCTTAGTG983030.5706197275156277No Hit
ATTCCTAACTGCTACA980510.569156942337821No Hit
CCATGATGATTCGGGC975770.5664055130748035No Hit
TCCGCGAGAGAACAGC963420.559236704762933No Hit
AGGTCGAGAAGGGAGG956580.5552662878517433No Hit
ACTGATTCTCGGCTCA951010.5520330682325434No Hit
TGGACCTCTAGGGAGG916550.5320300613963446No Hit
GCAATCATGGAACGTG882170.512073492184838No Hit
TGGCCTGGACCCGAGT842130.4888314610263528No Hit
TTCGATGGAAATCCTG813000.47192236093527695No Hit
ACGAGGATGGAGCTGA791310.45933195994058307No Hit
AAGGTGCGAACAGATA775020.4498760986126179No Hit
TTTCGTAACAGTACGG767690.445621251250188No Hit
TGGTCCTTGGAGAGGG735370.4268604508745075No Hit
TCCCAGACTGTCTTGA734440.4263206134874598No Hit
TCTCTCTACTCTGGAT723860.42017923762190607No Hit
GCGGTTCGAGTACGTT697260.40473872741172356No Hit
TTCGATGGACAGGTGA639850.3714139269919274No Hit
TCGCCGAGAAGGTAAT638340.37053741682586067No Hit
TGATATCCTCGATGTT635900.3691210692727462No Hit
TCCTTCACTTGTCACA633410.367675698204199No Hit
AGCTGCTCTTGTCACA629650.36549312984366195No Hit
CTTTCGGTGCTTTGTT619970.35987417725589627No Hit
AACTCCCTGCTTCACC613830.35631008956076393No Hit
AGCCCAGTGAAAGTAC567600.32947494719171366No Hit
TCGGCTTACCTCCTAC562490.3265087439144944No Hit
GGGGGGGGGGGGGGGG556590.3230839690934389No Hit
CCCTTGCCTCCTACGG555210.3222829200674971No Hit
AACCCTCACGCGGTTC547930.31805709622049977No Hit
ATGGCCGTGATACGCT545130.31643177935627004No Hit
AAACACGCTGCAGTTT535950.3111030619228311No Hit
TGTTTGTTGCAAGTTC534790.3104297163647931No Hit
CTAACTGGAACCTTGA504880.29306784943296754No Hit
CAATGTGTGGTTGACT497340.28869110330572034No Hit
TACAACGTGAGCAGGT496240.28805258596620154No Hit
TGGCCTTTGCGAAAGC488850.2837629103852523No Hit
AAATAGGACTCTATCT485150.28161517024323446No Hit
TCAAAGGCTCTTAGTG476040.2763270857314013No Hit
ACAGCCGACAAACATG474520.27544477086224806No Hit
CTTTCGGTGAGCCATT465320.270104444022636No Hit
ACCCTTGTGGGCCATC458150.2659424719095906No Hit
GGACATTTGGCCGCTA457240.26541424392871593No Hit
TGAACTGTGTGCTGGC455300.26428813152992814No Hit
AGTGTTCGATTCGCCT430220.249729936188899No Hit
CAGCCGATGTAATAGG423940.2460845826505551No Hit
GAGGTTGGAGGAATAT417470.24232893975356715No Hit
GATAAGAACATTGGTC390380.22660399909214443No Hit
GGCTTGCGAATACGCT378740.21984732469941798No Hit
GTGCTAATGGGAGTCA376750.21869218878519756No Hit
CGACGTTCTTGCTACA376440.21851224298951497No Hit
GTAAAGCCTGAAATCA366340.21264949287211488No Hit
TTGGGAGGAATCTGTG362620.2104901433239239No Hit
TGTACTGGAAATGCTC349560.2029092010929095No Hit
GGAACATCTTTACGAC344680.20007650598668053No Hit
AGGGACCCTAATCCTG333230.1934301209525982No Hit
GGGTGAAACCGACTAT329270.19113145853033045No Hit
TACGTGTGAAACGCAG323180.18759639435063077No Hit
CGGATGAGAACAGGCA323010.18749771439815968No Hit
TCTTGAACTCGTACAT319620.1855299200518244No Hit
TAACGAGCTTACTTTC316650.18380592323512357No Hit
TACCACCTGAAACATG314140.18234894276040334No Hit
AGCTTGTGAACCGTGC311070.18056689891283714No Hit
CATGATGTGCCTAGTA302180.17540651786890774No Hit
ACTTATCTGTCAGGTT301440.17497696984050418No Hit
AACTAGCACTGCCACG291790.16937543136199812No Hit
ACGTGTTGATCTTTGT272030.1579053380630054No Hit
TGGTAGGGATGTGCTA271600.15765573583028442No Hit
AACTACGACATTGCCA268540.15587949668580478No Hit
CGTCGTACTTGCGCCA264670.1536330765913158No Hit
GTAGATAGAGCTGAGA263010.15266949587895104No Hit
AACGAACCTGACACCC262940.1526288629573453No Hit
TCGGACCGAATGTCCA262800.15254759711413382No Hit
CTCGGATCTAGTACGG259940.15088745203138487No Hit
TTTAGGGACTGGGCAA252800.14674289402759902No Hit
GGTAGTTTGTCCAGCG252780.14673128462142598No Hit
AGTTTGCGAGCAGGAT251490.14598247792326297No Hit
CCTTCAGTGCAACGCC250450.14537878880226338No Hit
TACCACCTGTACTTTC246940.14334133801888965No Hit
TGAGGAGTGGTCCCAG242080.14052025231883375No Hit
CTTTAACTGTTCGCCT239050.13876142728361374No Hit
AGAACGCGACACAGTT230490.13379260144153995No Hit
CTTACATTGGAAGGCT222390.1290907919414468No Hit
CAGGCCATGTCGTAGT219450.1273842092340056No Hit
CGATCGGTGCGAGGAT218310.12672247308214063No Hit
GAAAGTCCTTATCTGG215890.1253177349351992No Hit
GGGATGTGATGCATGT215700.12520744557655505No Hit
GAGAAGAGATGCTACA215530.12510876562408393No Hit
CAAAGACCTGCTTTGA214390.12444702947221899No Hit
GCCATAATGACCGTTA210890.12241538339193181No Hit
TGCAATGACTCCGTAA208620.12109771579128842No Hit
AATTCTGCTTAGCGAG208120.12080748063696169No Hit
GCTGGACACATTGGTC204040.11843916177765548No Hit
CCGTTACTGTTGGTTG203960.11839272415296322No Hit
TCCTCATCTTTCTGGA203710.11824760657579984No Hit
GAAGGACACCCAGCTG201760.11711568947392556No Hit
AACTACGACGCTGTTA201100.11673257907021427No Hit
CCCACCTCTTGACCTA192870.11195530842999614No Hit
TGTCTTCCTGAAATCA191410.11110782177936207No Hit
GGACCACTGGCTAGAG189580.11004556111452622No Hit
TCGTAGAACCGCACTG188310.10930836382253628No Hit
ATAGCCAACATCTGTG188220.10925612149475748No Hit
ACAAGACCTAAGCTAA185740.10781655512929686No Hit
ATGGCCGTGGGCCAAA185430.10763660933361428No Hit
TCCGTAAGATTACGCA183810.10669624743359564No Hit
GGCTTAAACTGCATCA183690.10662659099655723No Hit
GCGTTTAGAAGTCTAC183620.10658595807495148No Hit
TATGGGTACCCTTTGC183300.10640020757618238No Hit
CCTGAGGACTGACTCA180360.10469362486874115No Hit
AGGTGATCTGTTAGTC178470.10359653598538607No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)