FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004950291

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004950291
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17227410
Sequences flagged as poor quality0
Sequence length34
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG397900.23096913581321857No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACACCG3700.012.1082075
TATACTG32600.09.619715
TAGACAG45300.09.1788835
CCTACAC23900.08.9028813
CTACGCT4100.08.8776834
AGGACCG6950.08.8633815
TACACTG38550.08.7160235
TAGACTG28450.08.4148315
GTCGTAG3003.430796E-88.4010441
CTAGGAC23700.08.3873523
GTATAGA22250.08.3695791
CTACGAC2851.3500176E-78.3500463
ACTGATC26550.08.3324148
CTAAGAC24400.08.3188453
TACACAG42950.08.214275
TAATCCG3251.5330443E-88.1846815
TTAGACG3251.5343176E-88.1842774
GACCGCG4205.0931703E-118.0008557
CTTAGAC20550.07.90189083
CTACACT30000.07.8863414
Produced by FastQC (version 0.11.5)