FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004950334

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004950334
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15340568
Sequences flagged as poor quality0
Sequence length34
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG17350.09.2753925
GTATTAG15000.08.7713881
TACACTG22900.08.7384515
TATACGG2602.9990224E-78.6115345
GTCCTAA16600.08.6005031
TCTAGAC15750.08.4392653
GTGTAGG21950.08.4172691
TACACCG7150.08.4158185
GTCTACG6500.08.3981381
CCTACAC23350.08.2690143
TAGACAG23850.08.2143565
GTCCTAG20300.08.0671771
TAGACTG16850.08.0557455
TAGACCG5750.08.0311925
CTTAGAC14500.08.0088783
TGCACCG18500.07.8667535
CTAGAAC21300.07.81679873
CTAGGAC20850.07.7841913
GTATAGA12100.07.75034761
CTAGACT18250.07.6669534
Produced by FastQC (version 0.11.5)