FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004950336

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004950336
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15340568
Sequences flagged as poor quality0
Sequence length34
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACCG2355.866241E-910.1269025
GTATTAG15600.09.3339431
TATACTG17900.09.2283615
CTAGTAC10650.08.8068823
ATACCGA3508.185452E-118.7993426
CCTATAC12050.08.713073
TACACTG26700.08.5986085
TAGACAG27600.08.5211025
TAGACTG20150.08.1284255
TACACCG6900.08.1153355
CTATACG2952.347042E-78.0671944
TAGTACC10300.08.0188184
GTATAGG12950.08.0005241
CCTACAC22050.07.8724533
GTGTTAG21400.07.7855561
ACACTGT35800.07.78154136
AGGACCG19450.07.7731985
CGTACAC6000.07.69944953
GTACCGT5400.07.51795676
TGTACTG19900.07.45672945
Produced by FastQC (version 0.11.5)