FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004950342

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004950342
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1469299
Sequences flagged as poor quality0
Sequence length8
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCAGTCAA138037893.94806639084352No Hit
TTAGTCAA657574.475399493227723No Hit
TCAGGCAA38290.2606004632140905No Hit
TCAGACAA34550.23514614792496286No Hit
TCAGTAAA29660.20186497098276115No Hit
ACAGTCAA26690.18165125001786567TruSeq Adapter, Index 13 (100% over 8bp)
TCAGTCAT16040.10916770514374542No Hit
TAAGTCAA15290.1040632301526102No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)