FastQCFastQC Report
Sun 4 Sep 2022
EGAF00004950352

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004950352
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3832801
Sequences flagged as poor quality0
Sequence length16
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCACTGTCTCTTTGTT392951.0252293296729988No Hit
TGCTGCTACCGTTCTC382050.9967905977899714No Hit
AAACGACCTCTTCAAG369180.9632120217042314No Hit
ATGGGTTACAATCGAA356460.9300248043141295No Hit
TATGCTTTGGACTGTT354450.9247805977925804No Hit
AAAGCGGACTCGGGCT332500.8675117753308873No Hit
GCAGGTCTGGCTGTTA326240.8511790724329282No Hit
TCCAAAGTGCTCACGG315340.8227403405499008No Hit
TAGTGCATGAACCCGC303630.792188271710428No Hit
TAACTCACTGGTGTCC287210.7493475398279221No Hit
GAAGTATGAACAGGTG287160.7492170869293763No Hit
TGTGGCTCTTACGATC281280.7338758260603668No Hit
TTCCCATCTCTTTGTT247460.6456374854838537No Hit
GCCTCTTGATCTGTGA243560.6354621593972659No Hit
TCACCTGACTCAATGC239270.6242693007020192No Hit
GGAACTACTACCAAGG237350.6192599093978529No Hit
ATGTCCAGAGTCGGTC232700.6071277898330751No Hit
TGAGAATGAGCAGTTT229990.600057242731882No Hit
CTAGAGACTAATCTGC227100.5925170651959233No Hit
GAAGTAATGAGTCTGT225970.5895688296887838No Hit
TTCGTCCGACCTGTGG221870.5788716920080119No Hit
TCGTATACTGCTAATC217990.5687485470808424No Hit
TGCGGTGACCTTAGTG217100.5664264854867237No Hit
TCACACAGATTGGGAG215460.562147630414415No Hit
ATTCCTAACTGCTACA207500.541379528965892No Hit
ACTGATTCTCGGCTCA206690.5392661920094468No Hit
TCCGCGAGAGAACAGC203590.531178112299595No Hit
CCATGATGATTCGGGC202710.5288821412851854No Hit
AGGTCGAGAAGGGAGG200030.5218898659231199No Hit
TGGACCTCTAGGGAGG196320.5122102608510068No Hit
TGGCCTGGACCCGAGT192960.5034438260687158No Hit
GCAATCATGGAACGTG191370.499295423894953No Hit
ACGAGGATGGAGCTGA173720.4532455507082157No Hit
TTCGATGGAAATCCTG170030.4436181267955211No Hit
AAGGTGCGAACAGATA164220.4284594999844761No Hit
TCCCAGACTGTCTTGA163530.4266592499845413No Hit
TTTCGTAACAGTACGG161340.42094541302822663No Hit
TGGTCCTTGGAGAGGG156050.40714349636206004No Hit
GCGGTTCGAGTACGTT154200.4023167391158581No Hit
TCTCTCTACTCTGGAT152220.3971508043334366No Hit
TCCTTCACTTGTCACA141690.36967742389964936No Hit
TCGCCGAGAAGGTAAT136750.3567886775233048No Hit
TTCGATGGACAGGTGA135540.3536317173784916No Hit
TGATATCCTCGATGTT134400.35065739129164286No Hit
AGCTGCTCTTGTCACA132480.34564799998747653No Hit
AACTCCCTGCTTCACC129910.33894272100221223No Hit
CTTTCGGTGCTTTGTT127350.3322635325966571No Hit
AGCCCAGTGAAAGTAC126410.3298110181039923No Hit
CCCTTGCCTCCTACGG120560.31454802897411055No Hit
AACCCTCACGCGGTTC120310.3138957644813806No Hit
TCGGCTTACCTCCTAC117130.3055989601338551No Hit
TGTTTGTTGCAAGTTC116070.30283335868468No Hit
ATGGCCGTGATACGCT115840.30223327535136835No Hit
CTAACTGGAACCTTGA112470.2934407499893681No Hit
AAACACGCTGCAGTTT111770.2916144094097241No Hit
AAATAGGACTCTATCT108760.283761144917255No Hit
TACAACGTGAGCAGGT107460.2803693695550591No Hit
CAATGTGTGGTTGACT105890.2762731485407147No Hit
TGGCCTTTGCGAAAGC105770.2759600615842044No Hit
TCAAAGGCTCTTAGTG105260.27462944201903516No Hit
ACAGCCGACAAACATG105080.27415981158426955No Hit
GGGGGGGGGGGGGGGG103140.2690982391206848No Hit
CTTTCGGTGAGCCATT99000.2582967391210762No Hit
GGACATTTGGCCGCTA98130.2560268586863758No Hit
ACCCTTGTGGGCCATC97040.2531829854980731No Hit
TGAACTGTGTGCTGGC95210.24840840941128955No Hit
CAGCCGATGTAATAGG95100.24812141303448837No Hit
AGTGTTCGATTCGCCT93620.2442600072375268No Hit
GAGGTTGGAGGAATAT91720.2393027970927789No Hit
GATAAGAACATTGGTC89030.2322844311510042No Hit
GGCTTGCGAATACGCT85770.22377890216580512No Hit
GTGCTAATGGGAGTCA84750.22111766303546673No Hit
CGACGTTCTTGCTACA81010.2113597862242261No Hit
GTAAAGCCTGAAATCA80620.2103422536155673No Hit
TTGGGAGGAATCTGTG78760.2054894057896562No Hit
TGTACTGGAAATGCTC78160.2039239710071042No Hit
GGAACATCTTTACGAC76900.20063655796374505No Hit
TCTTGAACTCGTACAT73010.19048732245686642No Hit
GGGTGAAACCGACTAT71770.18725209057292566No Hit
TAACGAGCTTACTTTC69470.18125125723980973No Hit
AGGGACCCTAATCCTG68920.17981627535580377No Hit
CGGATGAGAACAGGCA68800.17950318839929336No Hit
TACCACCTGAAACATG68100.17767684781964938No Hit
TACGTGTGAAACGCAG67010.17483297463134664No Hit
AACTAGCACTGCCACG66420.17329363042850385No Hit
CATGATGTGCCTAGTA65570.1710759311532219No Hit
ACTTATCTGTCAGGTT64620.16859732608084793No Hit
AGCTTGTGAACCGTGC64230.16757979347218913No Hit
ACGTGTTGATCTTTGT63660.16609263042876476No Hit
TCGGACCGAATGTCCA61900.16150068839994564No Hit
TGGTAGGGATGTGCTA60250.1571957427479277No Hit
AACTACGACATTGCCA59720.15581294202334012No Hit
GTAGATAGAGCTGAGA58700.15315170289300176No Hit
CTCGGATCTAGTACGG58470.15255161955969015No Hit
CGTCGTACTTGCGCCA57330.1495772934728414No Hit
AACGAACCTGACACCC56810.14822058332796303No Hit
TTTAGGGACTGGGCAA56070.14628988042948227No Hit
GGTAGTTTGTCCAGCG55830.14566370651646146No Hit
TACCACCTGTACTTTC55820.14563761593675226No Hit
TGAGGAGTGGTCCCAG55530.1448809891251855No Hit
CCTTCAGTGCAACGCC54680.1426632898499035No Hit
AGTTTGCGAGCAGGAT54370.14185448187891833No Hit
CTTTAACTGTTCGCCT52400.136714637676206No Hit
CAGGCCATGTCGTAGT51010.13308804709662725No Hit
CGATCGGTGCGAGGAT50820.13259232608215243No Hit
GAAAGTCCTTATCTGG50050.13058335144454408No Hit
AGAACGCGACACAGTT49550.1292788224590841No Hit
CTTACATTGGAAGGCT49510.1291744601402473No Hit
GAGAAGAGATGCTACA48860.12747857245914931No Hit
GCCATAATGACCGTTA48830.12740030072002173No Hit
GGGATGTGATGCATGT48800.12732202898089412No Hit
GAAGGACACCCAGCTG48640.12690457970554692No Hit
CAAAGACCTGCTTTGA47800.12471297100997417No Hit
TGCAATGACTCCGTAA46590.12155601086516102No Hit
AACTACGACGCTGTTA45710.11926003985075145No Hit
TCCTCATCTTTCTGGA44480.1160508985465199No Hit
AATTCTGCTTAGCGAG44130.11513772825669791No Hit
GCTGGACACATTGGTC44030.11487682245960591No Hit
GGCTTAAACTGCATCA43530.11357229347414594No Hit
GCGTTTAGAAGTCTAC43300.11297221014083435No Hit
CCGTTACTGTTGGTTG42940.11203294927130317No Hit
ATGGCCGTGGGCCAAA42250.11023269927136839No Hit
TGTCTTCCTGAAATCA41850.1091890760830004No Hit
ATAGCCAACATCTGTG41750.1089281702859084No Hit
TCGTAGAACCGCACTG41730.10887598912648999No Hit
CCCACCTCTTGACCTA41620.10858899274968882No Hit
CCTGAGGACTGACTCA41530.108354177532306No Hit
TCCGTAAGATTACGCA41010.10699746738742763No Hit
ACAAGACCTAAGCTAA40630.10600602535847804No Hit
TATGGGTACCCTTTGC40090.10459713405418125No Hit
AGGTGATCTGTTAGTC39680.10352742028610408No Hit
GGACCACTGGCTAGAG39630.10339696738755808No Hit
GCGTTAATGGTACGTT39280.1024837970977361No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)