FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004950358

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004950358
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18406450
Sequences flagged as poor quality0
Sequence length34
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGAGAC18850.012.03087622
ATCTCCG22300.010.546391510
CCCACGA24900.010.00710119
TCTCCGA23900.09.95747811
CACGAGA24500.09.94190621
CTTATAC26950.09.6134661
TATACAC40650.09.5771943
CTAGTAC12000.08.63583853
CTAGGAC28400.08.3827133
CCACGAG30700.08.25323820
ATACCGT4257.2759576E-128.2376846
TATACTG23350.08.1565425
TAGACTG25650.08.0257215
TTATACA38050.08.0233312
TCCGAGC31250.08.01865113
TAGGACC17750.07.96844344
TAGGACT27900.07.8803584
TCTATAC15900.07.8387523
TAGACAG34250.07.80956465
TACACAT43800.07.801355
Produced by FastQC (version 0.11.5)