FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004950361

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004950361
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18406450
Sequences flagged as poor quality0
Sequence length34
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG243240.13214932808879495No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACTG26450.09.7360745
GTATAGG16300.08.681591
TATACTG21800.08.6028055
TCTATAC17500.08.5571753
GTGTATA34700.08.2773231
CTAGACT24350.08.2768664
CTATACT19550.08.2329124
GTGTAGA26500.08.1950451
CTAGGAC28000.08.0973263
TACACCG7600.07.91856775
TAGGACC17150.07.83446744
TACACTG33700.07.8076395
GTGTAGG30550.07.75070141
TAGGACA24750.07.7472534
ACACCGT11400.07.7346466
CTAAGAC22700.07.70670653
GTATAGA17300.07.612851
TCTAGAC20800.07.6032593
GTGTTAG27250.07.50674871
GTAGGAC15950.07.4583533
Produced by FastQC (version 0.11.5)