FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004950375

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004950375
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2800954
Sequences flagged as poor quality0
Sequence length8
%GC62

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGAAGGG274018097.83023926847781No Hit
GAGAAGGA170220.6077215120276878No Hit
GAGCAGGG109000.3891531242569496No Hit
GAGAAGTG38160.1362392956114238No Hit
GAGAAGGC36750.1312052964811275TruSeq Adapter, Index 20 (100% over 8bp)
GAGAACGG30290.10814172599764223No Hit
GAGAAGCG29260.10446440748402151No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)