FastQCFastQC Report
Sun 4 Sep 2022
EGAF00004950376

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004950376
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3979896
Sequences flagged as poor quality0
Sequence length16
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCTGCTACCGTTCTC399591.0040212106044981No Hit
GCACTGTCTCTTTGTT395280.9931917818958084No Hit
AAACGACCTCTTCAAG384800.9668594355229383No Hit
ATGGGTTACAATCGAA375240.9428387073431065No Hit
TATGCTTTGGACTGTT371100.9324364254744345No Hit
AAAGCGGACTCGGGCT344800.86635429669519No Hit
GCCTCTTGATCTGTGA335150.8421074319529956No Hit
TCCAAAGTGCTCACGG323420.8126342999917586No Hit
GCAGGTCTGGCTGTTA322840.8111769754787562No Hit
TAGTGCATGAACCCGC314200.7894678654919626No Hit
TAACTCACTGGTGTCC297110.7465270449278072No Hit
TGTGGCTCTTACGATC292140.7340392814284594No Hit
GAAGTATGAACAGGTG280180.7039882449189627No Hit
TTCCCATCTCTTTGTT258850.6503938796390659No Hit
TCACCTGACTCAATGC252440.6342879311419193No Hit
GGAACTACTACCAAGG244250.6137095039669378No Hit
TGAGAATGAGCAGTTT241290.6062721236936844No Hit
ATGTCCAGAGTCGGTC239970.6029554541123688No Hit
TCGTATACTGCTAATC232750.5848142765539602No Hit
TTCGTCCGACCTGTGG232250.5835579623186133No Hit
CTAGAGACTAATCTGC231810.5824524057915081No Hit
GAGCCTGACCGAGTTT230680.5796131356196242No Hit
TGCGGTGACCTTAGTG225830.5674268875367597No Hit
GAAGTAATGAGTCTGT220940.5551401343150676No Hit
TCACACAGATTGGGAG220070.552954147545564No Hit
ATTCCTAACTGCTACA218310.5485319214371431No Hit
TCCGCGAGAGAACAGC217130.5455670198417245No Hit
ACTGATTCTCGGCTCA210460.5288077879421975No Hit
AGGTCGAGAAGGGAGG209320.5259433914856066No Hit
TGGCCTGGACCCGAGT208260.5232800053066713No Hit
TGGACCTCTAGGGAGG204390.5135561331250866No Hit
CCATGATGATTCGGGC196790.4944601567478145No Hit
GCAATCATGGAACGTG186030.46742427440315026No Hit
ACGAGGATGGAGCTGA181850.4569214873956505No Hit
TTCGATGGAAATCCTG175740.44156932743971194No Hit
AAGGTGCGAACAGATA174380.4381521527195686No Hit
TTTCGTAACAGTACGG167930.4219456990835942No Hit
TCCCAGACTGTCTTGA165720.416392790163361No Hit
TGGTCCTTGGAGAGGG161670.40621664485705156No Hit
TCTCTCTACTCTGGAT161560.4059402557252752No Hit
TCGCCGAGAAGGTAAT142820.35885359818447515No Hit
TCCTTCACTTGTCACA142410.3578234205114908No Hit
TGATATCCTCGATGTT141160.35468263492312363No Hit
TTCGATGGACAGGTGA139170.34968250426644315No Hit
AGCCCAGTGAAAGTAC138680.34845131631580323No Hit
AGCTGCTCTTGTCACA138550.34812467461461305No Hit
GCGGTTCGAGTACGTT136540.3430742913885187No Hit
CTTTCGGTGCTTTGTT136360.34262201826379385No Hit
AACTCCCTGCTTCACC136130.3420441137155343No Hit
CCCTTGCCTCCTACGG131420.3302096336185669No Hit
AACCCTCACGCGGTTC130600.3281492782725981No Hit
ATGGCCGTGATACGCT129440.3252346292465934No Hit
TCGGCTTACCTCCTAC122600.3080482505070484No Hit
GGGGGGGGGGGGGGGG121150.30440493922454254No Hit
TGTTTGTTGCAAGTTC120630.3030983724197818No Hit
AAACACGCTGCAGTTT117170.2944046779111816No Hit
ACCCTTGTGGGCCATC115120.28925378954625947No Hit
TGGCCTTTGCGAAAGC113550.28530896284727036No Hit
TACAACGTGAGCAGGT112080.28161539899535065No Hit
AAATAGGACTCTATCT111700.28066060017648703No Hit
CTAACTGGAACCTTGA111390.27988168535057195No Hit
CAATGTGTGGTTGACT109500.27513281754096086No Hit
TCAAAGGCTCTTAGTG105540.2651828087970138No Hit
ACAGCCGACAAACATG104090.26153949751450795No Hit
CTTTCGGTGAGCCATT102550.25767004966963963No Hit
TGAACTGTGTGCTGGC99180.2492024917234018No Hit
AGTGTTCGATTCGCCT97610.24525766502441268No Hit
CAGCCGATGTAATAGG97320.2445290027679115No Hit
GGACATTTGGCCGCTA94730.2380212950288148No Hit
GAGGTTGGAGGAATAT85820.21563377535493392No Hit
TTGGGAGGAATCTGTG84130.21138743323946152No Hit
CGACGTTCTTGCTACA83090.20877429962994007No Hit
TGTACTGGAAATGCTC81180.2039751792509151No Hit
GTGCTAATGGGAGTCA80570.20244247588379194No Hit
GGAACATCTTTACGAC77090.19369852880577784No Hit
AGGGACCCTAATCCTG75170.18887428214204594No Hit
TAACGAGCTTACTTTC74370.18686417936549096No Hit
GGACCACTGGCTAGAG73460.1845776874571597No Hit
CGGATGAGAACAGGCA72960.18332137322181283No Hit
TCTTGAACTCGTACAT72480.18211531155587984No Hit
GGGTGAAACCGACTAT72060.1810600075981885No Hit
TACCACCTGAAACATG71260.17904990482163352No Hit
TACGTGTGAAACGCAG70190.17636139235799125No Hit
AGCTTGTGAACCGTGC68180.17131100913189692No Hit
CATGATGTGCCTAGTA67720.17015520003537782No Hit
ACGTGTTGATCTTTGT66820.16789383441175346No Hit
ACTTATCTGTCAGGTT65580.16477817510809328No Hit
CTCGGATCTAGTACGG63180.1587478667784284No Hit
AACTAGCACTGCCACG62870.15796895195251334No Hit
TGGTAGGGATGTGCTA62080.1559839754606653No Hit
TCGGACCGAATGTCCA61820.15533069205828495No Hit
GTCCGAGTGGTCTTGA61290.15399899896881727No Hit
AACTACGACATTGCCA59980.15070745567220853No Hit
AACGAACCTGACACCC59180.14869735289565356No Hit
CGTCGTACTTGCGCCA58090.1459585878625974No Hit
GGTAGTTTGTCCAGCG58030.1458078301543558No Hit
GTAGATAGAGCTGAGA57840.14533043074492397No Hit
TTTAGGGACTGGGCAA57570.14465202105783667No Hit
TACCACCTGTACTTTC57300.14397361137074938No Hit
TGAGGAGTGGTCCCAG56430.14178762460124586No Hit
AGTTTGCGAGCAGGAT56260.14136047776122795No Hit
CCTTCAGTGCAACGCC56240.14131022519181405No Hit
CTTTAACTGTTCGCCT54060.13583269512570179No Hit
GCCATAATGACCGTTA53940.13553117970921852No Hit
CGATCGGTGCGAGGAT52660.1323150152667306No Hit
CTTACATTGGAAGGCT51800.130154154781934No Hit
AGAACGCGACACAGTT51250.12877220912305248No Hit
GTCCGTTACGGATGGT50840.12774203145006804No Hit
GGCTTGCGAATACGCT50180.12608369665941022No Hit
GGGATGTGATGCATGT50020.1256816761040992No Hit
CAAAGACCTGCTTTGA48950.12299316364045693No Hit
TGCAATGACTCCGTAA48530.12193785968276558No Hit
CCGTTACTGTTGGTTG48370.1215358391274546No Hit
CAGGCCATGTCGTAGT47370.11902321065676087No Hit
GAGAAGAGATGCTACA46560.11698798159549897No Hit
AATTCTGCTTAGCGAG46200.11608343534604923No Hit
TCCTCATCTTTCTGGA45430.1141487114236151No Hit
AACTACGACGCTGTTA45340.11392257486125265No Hit
CCCACCTCTTGACCTA45040.11316878632004455No Hit
GAACCATACTTTGGGT44990.11304315489650985No Hit
CCTGAGGACTGACTCA44390.11153557781409364No Hit
GAAAGTCCTTATCTGG43950.1104300212869884No Hit
TGTCTTCCTGAAATCA43830.11012850587050517No Hit
ATGGCCGTGGGCCAAA43360.10894757048927911No Hit
TCGTAGAACCGCACTG42600.1070379728515519No Hit
TCCGTAAGATTACGCA41700.10477660722792756No Hit
GAAGGACACCCAGCTG41500.10427408153378882No Hit
TATGGGTACCCTTTGC40930.10284188330549342No Hit
GATAAGAACATTGGTC40340.10135943250778412No Hit
AGGTGATCTGTTAGTC40240.10110816966071474No Hit
ACAAGACCTAAGCTAA40130.10083178052893846No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)