FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004950380

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004950380
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2566561
Sequences flagged as poor quality0
Sequence length16
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACAGCCGACCAGGGAT466451.8174124830853429No Hit
GAAGCAGTGGAACCTT141540.5514772491283083No Hit
GTTCTTCCTAACGTAA135380.5274762610356816No Hit
CGCTTAAGATAATAGG107340.41822501004262125No Hit
CATGGAGGATCGACAT107190.41764057039750857No Hit
TGTCCCATGAGACAAT104180.40591281485224784No Hit
AAAGCATGATCTTTGT98490.38374307098097415No Hit
TGTCGAAACTTCGCAA90250.35163785314278523No Hit
TGAGCGCACAGGACAC88620.34528694233256096No Hit
TAATTGCCTGTCTGGC85670.3337929626453453No Hit
GATACGTACCGCTCGA81990.3194547100185813No Hit
GGGGGGGGGGGGGGGG81300.31676628765106307No Hit
GGAAGGACTAGGTAAT78680.3065580751830952No Hit
AATGATGGAAAGCTAA71440.27834912164565734No Hit
CATGTCAGAGGTTTGT69470.2706734809731777No Hit
TCGACCTTGTTTCGCC61730.24051639528536434No Hit
GTGAGATCTCGATTAC57420.22372349614912718No Hit
GCTATGATGACCTGCG55160.21491793882942975No Hit
ATGGGTACTTGGCTGC52780.20564482979364215No Hit
AAACGACCTAGGGAGG50050.1950080282525917No Hit
CAGCTCACTGTTTACG49550.19305989610221616No Hit
TTGCGATGACATTCAG48010.1870596490790595No Hit
TGTTCCTCTTTATGGC47880.18655313471996185No Hit
AGCCTACCTTAACTCG47750.18604662036086422No Hit
CTGCCTTGACCATAAC44330.1727213964522955No Hit
CAATGTGACCAACGCC44070.17170836773410023No Hit
CCTGTTGACCCTAGTA43280.16863031893650687No Hit
CACAGCTCTGGACTGA43120.1680069166483867No Hit
AGCTTGTGACTCACTC43070.16781210343334915No Hit
TAACCAGACATCACAC41730.16259110927034268No Hit
CATGCAAACGAAGGCT41650.1622794081262826No Hit
ATGGGTACTTTCGGGC41150.16033127597590704No Hit
ATGCCGCGATGCCACG40970.15962994840177186No Hit
CATGATGTGTCATGTG40440.1575649283223738No Hit
CTATTCAACCGAACGA39980.15577264674402827No Hit
TTGTTTGTGATACGCT39350.1533180002345551No Hit
CATTAGTCTATTCTGG38800.151175054869142No Hit
ATTAGCGTGGTCGGTC38120.1485255951446313No Hit
AGAACGCGAGGAATAT37540.14626576185019566No Hit
CACTATACTACGCCAC36760.1432266756956098No Hit
GCATCTCTGAGCCTAT35700.13909663553681367No Hit
GCAGATACTCGGCTCA35250.13734331660147567No Hit
ATAGGAAACAGACTTC35210.13718746602944562No Hit
CCCAATGCTGGAATGC34740.13535622180809262No Hit
AACGTCCCTTACAGAA34550.1346159315909499No Hit
GGGTCGTACCGCTCGA34400.13403149194583724No Hit
AGCTCGGACCTTAAGC34330.1337587534447847No Hit
CTGCCATTGCACAGTT34050.13266779944057439No Hit
CCGCTATTGTTCGCAA33980.1323950609395218No Hit
GGGATCAGATGAGTAA33670.13118721900628896No Hit
AACCAACACCGGAATG33440.13029107821711622No Hit
ATAGCCAACATGGCCC33270.12962871328598854No Hit
CTGATTACTGCTTTGA33260.12958975064298103No Hit
TTGACGTGACCTCAAA33100.12896634835486084No Hit
TTGTAACCTCATCCTC32790.127758506421628No Hit
CCTACGGGATGGAACG32750.12760265584959796No Hit
TGGGCCATGCGTTCTC32730.12752473056358293No Hit
CGCTTACTGGCCACGT32170.12534282255516233No Hit
CGATCAAACCGATCGC32090.12503112141110226No Hit
TGGAGTTACAAGTCTC31560.12296610133170416No Hit
TGTTTGTTGCGCTCGA31240.12171929675546383No Hit
ATCTTACTGCGCACTG31180.12148552089741876No Hit
GATACCATGTCTGGAT30920.12047249217922348No Hit
TCAGGCTCTTAAAGCA30450.11864124795787047No Hit
TCCAAAGTGAAGGCCA30340.11821265888478785No Hit
TTCTAACACTACGTTG30140.11743340602463764No Hit
CGCTGAGTGTTAGCTG29820.11618660144839729No Hit
TGGGTGCACTTCTGAG29680.11564112444629214No Hit
GTAAGAAACGACAAAT29630.11544631123125458No Hit
GCGTCGATGCTTGAAC29610.11536838594523957No Hit
CATGTTACTAACGTGG29550.1151346100871945No Hit
CGCTTCCCTGCGCTGT29550.1151346100871945No Hit
TACAACCGATTCCATT29490.11490083422914944No Hit
CGAGTCTCTTACTTTC29290.11412158136899922No Hit
CTCGGATCTTTCTGAG29010.1130306273647889No Hit
AGTGGTACTCAACGCC28570.11131627107245845No Hit
TACGCCTTGGGTTGAG28390.11061494349832324No Hit
GTGTACTGAATCACAC28370.11053701821230823No Hit
CTTAGGCCTTATGACC28330.11038116764027818No Hit
ATTGAGAGATGATCAC28250.1100694664962181No Hit
CCTCCCTTGTTTCGCC28060.10932917627907539No Hit
AGAACAATGAGTCGGA28040.10925125099306038No Hit
CCTTCAGTGTAGCTTG28030.10921228835005284No Hit
GCATAGTACGCTCCTT27910.10874473663396274No Hit
TATCTCCCTGGAATTA27800.10831614756088011No Hit
CATGTCAGAACAGGTG27720.10800444641682003No Hit
TGAGATAGACTCATCC27710.10796548377381252No Hit
ATCGCAATGTAACTCG27570.10742000677170735No Hit
GCATATCGAGCCGCTA27400.10675764184057968No Hit
TTCACGCTGTGGGCAA27300.10636801541050456No Hit
ACCTCCATGGAATTGA27060.10543291197832431No Hit
GCGACAAGAAAGGCTG26860.10465365911817408No Hit
AACAGCTACTCGAGAG26770.10430299533110649No Hit
AACCACTGAAAACATG26650.10383544361501636No Hit
TTCTATGACCAACGCC26480.10317307868388868No Hit
TGGTCCTTGTTTCTGT26410.10290034018283611No Hit
AGGTTACTGGTCCCGA26320.1025496763957685No Hit
AGAACATGAAAGCTAA26310.102510713752761No Hit
GGTAGCCTGTGCCACG26190.10204316203667085No Hit
GGTCAGAGAACCAGTT26130.10180938617862578No Hit
AGAGAATTGCCCGCAA25910.10095220803246056No Hit
GGGCTGACTAATACAG25890.10087428274644554No Hit
ATTCCTAACATTGCCA25860.100757394817423No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)